Therapeutic effect of Guijiajiao (Colla Carapacis et Plastri) on bone regeneration in rats and zebrafish

IF 2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine Pub Date : 2018-04-01 DOI:10.1016/j.jtcm.2018.04.006

Su Weiyang , Feng Chienwei , Tseng Chungchih , Hung Hanchun , Chen Zhicheng , Lee Hsinpai , Jean Yenhsuan , Wu Shingyi Sean , Wen Zhihong

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引用次数: 5

Abstract

OBJECTIVE

To investigate the effect of Guijiajiao (Colla Carapacis et Plastri, CCP), the glue of tortoise shell, on bone regeneration in zebrafish and male Wistar rats.

METHODS

In this study, we applied in vitro and in vivo models, tissue section analysis, and quantitative polymerase chain reaction to assess the effects of CCP on bone repair. MG-63 cells were used in alkaline phosphatase (ALP) activity and mineralization assays. Zebrafish and male Wistar rats were used to evaluate the effects of CCP on bone repair in vivo.

RESULTS

A simple preparation of CCP promoted osteogenesis in vivo and in vitro, and promoted MG-63 cell proliferation as well as ALP activity and mineralization. In addition, CCP activated Akt and extracellular signaling-regulated kinase pathways and significantly increased the expression of ossification-related genes and proteins such as runt-related transcription factor-2, osteocalcin, and osteopontin. The in vivo results revealed promotion of osteogenesis by immersing zebrafish in CCP for 72 h. An oral dose of 1.25 g/kg CCP significantly improved skull defects in rats, which was accompanied by an increase in serum ALP levels.

CONCLUSION

One of the ingredients of Guilu Erxianjiao (tortoise shell and deer horn formula) provides a practical alternative therapy for bone regeneration.

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归甲胶对大鼠和斑马鱼骨再生的治疗作用

目的探讨龟甲胶龟甲胶对斑马鱼和雄性Wistar大鼠骨再生的影响。方法采用体外、体内模型、组织切片分析、定量聚合酶链反应等方法评价CCP对骨修复的作用。MG-63细胞用于碱性磷酸酶(ALP)活性和矿化测定。采用斑马鱼和雄性Wistar大鼠在体观察CCP对骨修复的影响。结果单纯制备CCP可促进体内、体外成骨，促进MG-63细胞增殖、ALP活性和矿化。此外，CCP激活了Akt和细胞外信号调节激酶通路，显著增加了骨化相关基因和蛋白的表达，如矮子相关转录因子-2、骨钙素和骨桥蛋白。体内实验结果显示，将斑马鱼浸泡在CCP中72小时可促进成骨。口服1.25 g/kg CCP可显著改善大鼠颅骨缺损，并伴有血清ALP水平升高。结论龟鹿二仙椒(龟甲鹿角方)是一种实用的骨再生替代疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.40

自引率

3.80%

发文量

32269

审稿时长

2 months

期刊介绍： Journal of Traditional Chinese Medicine（JTCM） is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.