Mapping and identification of QTL for agro-physiological traits in rice (Oryza sativa L.) under drought stress

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2023-03-01 DOI:10.1016/j.plgene.2022.100397

Rizky Dwi Satrio , Miftahul Huda Fendiyanto , Ence Darmo Jaya Supena , S. Suharsono , M. Miftahudin

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引用次数: 1

Abstract

Exploring novel QTLs for drought tolerance traits using a new rice genetic resource would be valuable to dissect the mechanisms underlying the complexity of the trait. Here, we used a recombinant inbred line population to detect the QTL associated with agro-physiological traits under drought stress, in particular incorporating the SNPs and 147 phenotype data, analyzed the transcript expression of genes within QTL using differential gene expression meta-analysis and qRT-PCR technique. Composite interval mapping analysis allowed the detection of 154 QTLs distributed into 66 regions, which included a large QTL cluster called ‘hotspot QTL’ that strongly associated with drought tolerance on rice chromosome 8. We found several genes within the QTL-containing regions that were highly expressed based on the meta-analysis approach. In the future, the QTL reported here may be utilized for marker-assisted breeding and the candidate drought-responsive genes could be characterized for dissecting a comprehensive molecular mechanism of drought tolerance in rice.

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干旱胁迫下水稻农业生理性状QTL定位与鉴定

利用一种新的水稻遗传资源，探索抗旱性状的新QTL，对于揭示该性状复杂性的机制具有重要意义。在这里，我们使用重组自交系群体来检测干旱胁迫下与农业生理性状相关的QTL，特别是结合SNPs和147表型数据，使用差异基因表达荟萃分析和qRT-PCR技术分析QTL内基因的转录表达。通过复合区间定位分析，共检测到分布在66个区域的154个QTL，其中包括一个称为“热点QTL”的大型QTL簇，该簇与水稻8号染色体的耐旱性密切相关。基于荟萃分析方法，我们在含有QTL的区域中发现了几个高表达的基因。未来，本文报道的QTL可用于标记辅助育种，候选抗旱基因可用于剖析水稻抗旱性的综合分子机制。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.