Jingyuan Zhang, Dongqing Yang, Juan Zhao, Yun Ling, Chunxiang Zhou
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引用次数: 0
Abstract
Alzheimer’s disease is a common neurodegenerative disorder. Ling-Gui-Zhu-Gan decoction has anti-inflammatory, anti-tumor, and immune-regulating effects and is a representative agent for strengthening the spleen and promoting water retention. The present study aimed to investigate the underlying mechanism of Ling-Gui-Zhu-Gan decoction in alleviating Alzheimer’s disease. Alzheimer’s disease in vitro and in vivo models were constructed by exposing SH-SY5Y cells to β-amyloid 1-42 and injecting β-amyloid 1-42 into Sprague-Dawley rats, respectively. The Morris water maze test was used to assess cognitive decline in rats. Cell counting kit-8, flow cytometry analysis, and enzyme-linked immunosorbent assay were conducted to determine cell viability, apoptosis, and inflammation. β-amyloid 1-42 treatment induced SH-SY5Y cell apoptosis, evoked inflammation response and oxidative stress while hindering cell proliferation. Ling-Gui-Zhu-Gan decoction antagonized the effect of β-amyloid 1-42 on SH-SY5Y cells by activating the nuclear factor erythroid 2-related factor 2/antioxidant response element signaling pathway. In addition, Ling-Gui-Zhu-Gan decoction improved memory and learning abilities and alleviated neuropathologic lesions, inflammation response, and neurotoxicity in Alzheimer’s disease rats in a dose-dependent manner by upregulating nuclear factor erythroid 2-related factor 2/antioxidant response element pathway. The Ling-Gui-Zhu-Gan decoction improved the spatial learning and memory ability of Alzheimer’s disease rats by upregulating the nuclear factor erythroid 2-related factor 2/antioxidant response element signaling pathway to counteract the imbalance in the body’s oxidative stress levels and the damage caused by neuroinflammation.
期刊介绍:
Current Topics in Nutraceutical Research is an international, interdisciplinary broad-based peer reviewed scientific journal for critical evaluation of research on chemistry, biology and therapeutic applications of nutraceuticals and functional foods. The major goal of this journal is to provide peer reviewed unbiased scientific data to the decision makers in the nutraceutical and food industry to help make informed choices about development of new products.
To this end, the journal will publish two types of review articles. First, a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, a critical evaluation of current human experimental data to help market and deliver the product for medically proven use. This journal will also serve as a forum for nutritionists, internists, neurologists, psychiatrists, and all those interested in preventive medicine.
The common denominator of all of the topic to be covered by the journal must include nutraceuticals and/functional food. The following is an example of some specific areas that may be of interest to the journal. i) Role of vitamins, minerals, antioxidants and phytonutrients on cardiovascular health, cancer, diabetes, ocular health, mental health, men’s health, women’s health, infant nutrition, ii) Role of herbals on human health, iii) Dietary supplements and sleep, iv) Components of diet that may have beneficial effect on human health, v) regulation of apoptosis and cell viability, vi) Isolation and characterization of bioactive components from functional foods, vii) Nutritional genomics, and viii) Nutritional proteomics.