Characterization and functional significance of bacteria associated with rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) reared on guava plants

IF 1.3 4区农林科学 Q2 ENTOMOLOGY

Applied Entomology and Zoology Pub Date : 2022-07-06 DOI:10.1007/s13355-022-00791-9

M. Saranya, J. S. Kennedy, R. Anandham, A. Manikandan

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Abstract

Rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) is an introduced phloem feeder of guava. Gut symbiotic bacteria alter the physiology of the host insect. The present study characterized the nine bacterial isolates from the second nymphal stage of rugose spiralling whitefly using seven different bacteria growth media viz., Nutrient Agar (NA), Luria Bertani agar (LBA), MacConkey agar (MCA), Tryptic Soya Agar (TSA), Endo Agar (EA), R2A agar and MRS agar. Among the nine bacterial isolates, chitinase-producing isolates (seven species) were proceeded for biochemical tests, protease and siderophore activities. Out of seven chitinase producers, protease (seven species) and siderophore (two species) producers were identified using skim milk agar and Chromazurol S (CAS) agar plates, respectively. The biochemical tests showed that all bacterial isolates were Gram positive. Bacterial isolates SGRSW2, SGRSW4, SGRSW6 and SGRSW8 showed negative for carbohydrate fermentation, indole production, citrate utilization, starch hydrolysis tests and also, SGRSW4 showed positive for lactose fermentation. 16S rRNA gene sequencing results revealed that Bacillus genus alone is associated with rugose spiralling whitefly which includes Bacillus albus, Bacillus tequilensis and Bacillus altitudinis. Bacterial communities associated with rugose spiralling whitefly offers basic knowledge about their functional significance to study the physiological role in the host insect.

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在番石榴上饲养的皱纹螺旋粉蝶Aleurodicus rugioperculatus Martin（半翅目：Aleyrodidae）相关细菌的特征及其功能意义

白蝇(Aleurodicus rugioperculatus Martin，半翅目:白蝇科)是番石榴引种韧皮部食用者。肠道共生细菌改变宿主昆虫的生理机能。本研究采用营养琼脂(NA)、Luria Bertani琼脂(LBA)、MacConkey琼脂(MCA)、Tryptic Soya琼脂(TSA)、Endo琼脂(EA)、R2A琼脂和MRS琼脂7种不同的细菌生长培养基，对红毛螺旋体白蝇二稚期分离的9株细菌进行了鉴定。对9株产几丁质酶菌株(7种)进行生化、蛋白酶和铁载体活性测定。7个几丁质酶产生菌中，蛋白酶产生菌(7种)和铁载体产生菌(2种)分别用脱脂乳琼脂和Chromazurol S (CAS)琼脂平板进行鉴定。生化试验结果显示所有分离菌均为革兰氏阳性。分离菌株SGRSW2、SGRSW4、SGRSW6和SGRSW8对碳水化合物发酵、吲哚生产、柠檬酸利用和淀粉水解试验均呈阴性，SGRSW4对乳糖发酵呈阳性。16S rRNA基因测序结果显示，只有芽孢杆菌属(Bacillus genus)与皱毛螺旋体粉虱有亲缘关系，包括白色芽孢杆菌(Bacillus albus)、龙基芽孢杆菌(Bacillus tequilensis)和高原芽孢杆菌(Bacillus altitinis)。与皱毛螺旋粉虱相关的细菌群落为研究其在寄主昆虫中的生理作用提供了基本的功能意义。

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来源期刊

Applied Entomology and Zoology 生物-昆虫学

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.