Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Pub Date : 2018-06-25 DOI:10.5511/PLANTBIOTECHNOLOGY.18.0424A

Kanna Sato-Izawa, Kyoko Tokue, H. Ezura

引用次数: 4

Abstract

Sorghum is a recalcitrant crop for Agrobacterium-mediated genetic transformation. Several parameters related to Agrobacterium-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using Agrobacterium strain GV2260. Pretreatment of immature embryos with heat (43°C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% of immature embryos treated. Although further optimization to enhance transformation efficiency is required, this study contributes to the genetic validation of genes of interest and molecular breeding in sorghum plants.

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高粱双色Tx430稳定农杆菌介导转化方案的建立。

高粱是一种对农杆菌介导的遗传转化具有抗性的作物。对农杆菌介导转化的几个相关参数进行了测试，以优化高粱的转化频率。本研究利用农杆菌菌株GV2260对高粱品种Tx430未成熟胚进行预处理。将未成熟胚胎加热(43°C)处理15或21分钟，然后离心，使未成熟胚胎的转化效率高达1.9%。虽然还需要进一步优化以提高转化效率，但本研究有助于相关基因的遗传验证和高粱植株的分子育种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-PLANT SCIENCES

CiteScore

2.90

自引率

18.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.