Monitoring autophagy in rice tapetal cells during pollen maturation.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-06-25 DOI:10.5511/PLANTBIOTECHNOLOGY.19.0417A

Shigeru Hanamata, Jumpei Sawada, B. Toh, Seijiro Ono, K. Ogawa, Togo Fukunaga, K. Nonomura, Takamitsu Kurusu, K. Kuchitsu

引用次数: 9

Abstract

We have previously shown that autophagy is required for post meiotic anther development including programmed cell death-mediated degradation of the tapetum and pollen maturation in rice. However, the spatiotemporal dynamics of autophagy in the tapetum remain poorly understood. We here established an in vivo imaging technique to analyze the dynamics of autophagy in rice tapetum cells by expressing green fluorescent protein-tagged AtATG8, a marker for autophagosomes. 3D-imaging analysis revealed that the number of autophagosomes/autophagy-related structures is extremely low at the tetrad stage (stage 8), and autophagy is dramatically induced at the uninucleate stages (stage 9-10) throughout the tapetal cells during anther development. The present monitoring system for autophagy offers a powerful tool to analyze the regulation of autophagy in rice tapetal cells during pollen maturation.

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在花粉成熟过程中监测水稻绒毡层细胞的自噬。

我们之前已经表明，水稻减数分裂后花药发育需要自噬，包括程序性细胞死亡介导的绒毡层降解和花粉成熟。然而，对绒毡层中自噬的时空动力学仍知之甚少。我们在此建立了一种体内成像技术，通过表达绿色荧光蛋白标记的AtATG8（一种自噬体的标记物）来分析水稻绒毡层细胞的自噬动力学。3D成像分析显示，自噬体/自噬相关结构的数量在四分体阶段（第8阶段）极低，并且在花药发育过程中，整个绒毡层细胞在单核阶段（第9-10阶段）显著诱导自噬。目前的自噬监测系统为分析花粉成熟过程中水稻绒毡层细胞的自噬调控提供了一个强大的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.