Development and validation of RP-HPLC method for determination of amoxicillin residues and application to NICOMAC coating machine

Abstract

AMO, is a semi-synthetic penicillin antibacterial drug derived from a fermentation product. Chemically, it is (2S,5R,6R)-6-[[(2R)2-Amino-2-(4-hydroxyphenyl) acetyl] amino]-3,3-dimethyl-7-oxo-4thia-1-azabicyclo [3.2.0] heptane-2-carboxylic acid trihydrate having a molecular formula of C16H19N3O5S.3H2O and its molecular weight is 419.4. AMO is a crystalline powder with white or almost white appearance. It is slightly soluble in water, very slightly soluble in ethanol (96%), practically insoluble in fatty oils. It dissolves in dilute acids and dilute solutions of alkali hydroxides.