Prevalence of Plasmid-Mediated Quinolone Resistance Genes and Molecular Typing of Klebsiella pneumoniae Isolates from Blood Cultures in Milad Hospital, Tehran, Iran, Within 2018 - 2019

IF 0.5 4区医学 Q4 MICROBIOLOGY

Jundishapur Journal of Microbiology Pub Date : 2022-08-17 DOI:10.5812/jjm-124054

M. Ghane, L. Babaeekhou, Morteza Asgharifard

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引用次数: 1

Abstract

Background: Plasmid-mediated quinolone resistance (PMQR) determinants are commonly characterized in Klebsiella pneumoniae isolates worldwide and complicate the treatment of infections caused by this bacterium. Objectives: This study aimed to investigate the prevalence of PMQR determinants and molecular typing of blood isolates of K. pneumoniae in Milad hospital in Tehran, Iran, within 2018 - 2019. Methods: A total of 100 K. pneumoniae isolates were tested for susceptibility to quinolones using the disk diffusion method. The minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) and levofloxacin (LEV) were determined using the microdilution broth method. The PMQR determinants were detected by polymerase chain reaction (PCR) assay, and the genetic relationship between the isolates was assessed using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: The resistance rates of the isolates to LEV, CIP, nalidixic acid, and norfloxacin were determined to be 62%, 46%, 29%, and 23%, respectively. Eighty-one isolates were resistant to at least one tested quinolone. A high-level CIP and LEV resistance (MIC > 32 mg/L) was observed in 15 (18.51%) and 36 (44.44%) isolates, respectively. The PMQR genes were detected in 71 (87.65%) isolates. The oqxAB, qnrS, qnrD, qnrB, aac(6')-Ib-cr, qnrA, qepA, and qnrC genes were detected in 71 (87.65%), 30 (37%), 25 (30.8%), 24 (29.6%), 18 (22.2%), 17 (21%), 17 (21%), and 8 (9.9%) isolates, respectively. The ERIC-PCR revealed 64 genotypes among quinolone-resistant isolates. Conclusions: The high prevalence of PMQR genes observed in this study is a significant concern for public health since they can contribute to the spread of fluoroquinolone resistance among clinical isolates. The ERIC-PCR revealed high heterogeneity among the studied isolates, indicating that they emerged from different sources.

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2018 - 2019年伊朗德黑兰Milad医院血培养肺炎克雷伯菌质粒介导喹诺酮类耐药基因的流行及分子分型

背景：质粒介导的喹诺酮耐药性（PMQR）决定因素在世界各地的肺炎克雷伯菌分离株中普遍存在，并使该细菌引起的感染的治疗复杂化。目的：本研究旨在调查2018-2019年伊朗德黑兰Milad医院肺炎克雷伯菌血液分离株PMQR决定因素和分子分型的流行情况。方法：采用纸片扩散法检测100株肺炎克雷伯菌对喹诺酮类药物的敏感性。采用微量稀释液法测定了环丙沙星（CIP）和左氧氟沙星（LEV）的最低抑菌浓度（MIC）。通过聚合酶链式反应（PCR）检测PMQR决定簇，并使用肠道细菌重复基因间一致性（ERIC）-PCR评估分离株之间的遗传关系。结果：分离株对LEV、CIP、萘啶酸和诺氟沙星的耐药率分别为62%、46%、29%和23%。81个分离株对至少一种测试的喹诺酮类药物具有耐药性。在15个（18.51%）和36个（44.44%）分离株中分别观察到高水平的CIP和LEV抗性（MIC>32mg/L）。在71株（87.65%）分离株中检测到PMQR基因。oqxAB、qnrS、qnrD、qnrB、aac（6'）-Ib-cr、qnrA、qepA和qnrC基因分别在71个（87.65%）、30个（37%）、25个（30.8%）、24个（29.6%）、18个（22.2%）、17个（21%）、17（21%）和8个（9.9%）分离株中检测到。ERIC-PCR在喹诺酮类耐药菌株中揭示了64种基因型。结论：本研究中观察到的PMQR基因的高患病率是公众健康的一个重大问题，因为它们可能导致临床分离株对氟喹诺酮耐药性的传播。ERIC-PCR揭示了所研究分离株之间的高度异质性，表明它们来自不同的来源。

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来源期刊

Jundishapur Journal of Microbiology MICROBIOLOGY-

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.