The Effect of Apocynin on Motor and Cognitive Functions in Experimental Alzheimer’s disease

IF 0.2 4区生物学 Q4 BIOLOGY

Periodicum Biologorum Pub Date : 2018-06-30 DOI:10.18054/PB.V120I1.5975

Y. Akyuva, C. Onal, H. Parlakpınar, M. Gul, Y. Cigremis, Tuncay Ateş, Y. Kablan

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引用次数: 0

Abstract

Scope: We investigated the potential beneficial effect of Apocynin (APO) on motor and cognitive functions in experimental Alzheimer’s disease (AD). Materials and Methods: Experimental AD was induced in rats by intraventricular streptozotocin (STZ) injection. Sham group received artificial cerebrospinal fluid (CSF). Both groups were randomly divided into two subgroups. One of the subgroups received intraperitoneal APO for while the other had normal saline (NS). The animals were evaluated with rotarod, accelerod and Water-Maze tests before and after the treatment. Additionally, biochemical markers of oxidative stress such as malondialdehyde (MDA) and reduced glutathione (GSH) were analyzed from brain specimens. Standard histological evaluation and transmission electron microscopy (TEM) were used to evaluate the neural damage. Results: The difference between STZ+NS in comparison with CSF+NS, CSF+APO and STZ+APO were statistically significant on 30 and 40 rpm on rotarod test. GSH levels, accelerod and Water-Maze test results were not statistically significant between subgroups. However, MDA differences between STZ+NS in comparison with CSF+NS, CSF+APO and STZ+APO were statistically significant. Hemotoxilene eozine staining and TEM results showed apocynins protective effect. Conclusion: These results indicate that APO can provide neuro-protective effect for motor but not for cognitive performance in experimental AD. Keywords: Alzheimer’s disease, Streptozotocin, Apocynin, Rotarod test, Accelerod test, Water-Maze test

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罗布麻素对实验性阿尔茨海默病运动和认知功能的影响

范围:我们研究了罗布麻苷(APO)对实验性阿尔茨海默病(AD)的运动和认知功能的潜在有益作用。材料与方法:采用脑室注射链脲佐菌素(STZ)诱导大鼠实验性AD。假手术组给予人工脑脊液治疗。两组随机分为两个亚组。其中一个亚组给予APO腹腔注射，另一个亚组给予生理盐水(NS)。在给药前后分别进行旋转、加速和水迷宫试验。此外，还分析了脑标本中氧化应激的生化标志物，如丙二醛(MDA)和还原性谷胱甘肽(GSH)。采用标准组织学检查和透射电镜(TEM)评价神经损伤。结果:在30rpm和40rpm时，STZ+NS与CSF+NS、CSF+APO和STZ+APO比较，差异均有统计学意义。GSH水平、加速和水迷宫测试结果在亚组间无统计学意义。但STZ+NS与CSF+NS、CSF+APO、STZ+APO的MDA差异均有统计学意义。血氧苯乙胺染色和透射电镜结果显示罗布麻素有保护作用。结论:APO对实验性AD的运动功能有神经保护作用，但对认知功能无保护作用。关键词:阿尔茨海默病，链脲佐菌素，罗布麻素，Rotarod试验，Accelerod试验，水迷宫试验

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来源期刊

Periodicum Biologorum 生物-生物学

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.