Green solvents-based rutin extraction from Sophora japonica L.

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2023-09-01 DOI:10.1016/j.jarmap.2023.100508

Nhan Trong Le , Trieu Phat Dac Nguyen , Duc Viet Ho , Huong Thanh Phung , Hoai Thi Nguyen

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Abstract

Using green solvents to extract bioactive ingredients from medicinal herbs is a new trend for sustainable development procedures. The main objective of this study was to evaluate the ability to extract rutin from Sophora japonica L. using deep eutectic solvents (DESs), recover the target compound from extract, and reuse the solvent. Additionally, the antioxidant activity and cytotoxicity of the DESs extracts were also assessed. DESs of choline chloride and ethylene glycol (ChCl-Eth) showed the highest extraction efficiency of rutin from Sophora japonica L. The main factors affecting extraction yields were optimized by the response surface methodology (RSM). Under optimized conditions, the extraction yields using DESs and methanol as solvents were 26.20 % and 19.49 %, respectively. Water (as an antisolvent) was used to recover rutin from the DESs extract with a recovery yield of 94.9 %. The DESs solvent was recovered and recycled at least three times without loss of extraction yields. The DESs of ChCl-Eth had no significant effect on the antioxidant activity of rutin and the recovered rutin has exhibited more antioxidant activity than methanol extract. The selected greens solvents and their extracts did not show cytotoxic activity against the human embryonic kidney cell line (HEK-293A). The results serve as a basis for the development of other studies, as a premise to deploy a safe and environmentally friendly process of extracting natural active ingredients on an industrial scale.

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绿色溶剂法从槐花中提取芦丁。

利用绿色溶剂提取药材中的生物活性成分是可持续发展的新趋势。研究了深共晶溶剂(deep共晶溶剂，DESs)对槐花芦丁的萃取性能，并对提取物中的目标化合物进行回收和溶剂再利用。此外，还评估了DESs提取物的抗氧化活性和细胞毒性。采用氯化胆碱-乙二醇(ChCl-Eth)双溶剂法提取槐花中芦丁的提取率最高，并利用响应面法对影响提取率的主要因素进行了优化。在优化条件下，以DESs和甲醇为溶剂的提取率分别为26.20%和19.49%。以水为抗溶剂，从芦丁提取物中提取芦丁，回收率为94.9%。在不损失萃取率的情况下，将DESs溶剂回收并循环使用至少三次。ChCl-Eth的DESs对芦丁的抗氧化活性无显著影响，得到的芦丁的抗氧化活性高于甲醇提取物。所选绿色溶剂及其提取物对人胚胎肾细胞株HEK-293A无细胞毒活性。该研究结果为其他研究的发展奠定了基础，为在工业规模上部署安全环保的天然活性成分提取工艺提供了前提。

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来源期刊

Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

6.40

自引率

7.70%

发文量

审稿时长

41 days

期刊介绍： JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.