Simultaneous determination of three naphthoquinones from Arnebia euchroma (Royle) Johnst in beagle plasma by UPLC-MS/MS and application for pharmacokinetics study

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Acta Chromatographica Pub Date : 2021-12-14 DOI:10.1556/1326.2021.00982

Zhibin Wang, Mingyu Zhang, Wenbo Zhu, Chunjuan Yang, H. Kuang

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引用次数: 0

Abstract

A rapid, simple and efficient ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method was established to simultaneous determination of shikonin, isobutyryl shikonin, β, βʹ-dimethylacryl alkanin in beagle plasma and evaluated by using esculetin as internal standard. The electrospray ionization (ESI) source was operated in negative ionization mode. Multi-reaction monitoring (MRM) was used to quantitatively analyzed shikonin m/z 287.0 → 217.9, isobutyryl shikonin m/z 357.0 → 268.9, β, βʹ-dimethylacryl alkanin m/z 370.0 → 270.1 and esculetin m/z 177.0 → 89.0, respectively. The method was validated for selectivity, linearity, lower limit of quantification, precision, accuracy, recovery, matrix effect and stability. All validation parameters met the acceptance criteria according to regulatory guidelines. This method was successfully applied for the pharmacokinetic study of shikonin, isobutyryl shikonin, β, βʹ-dimethylacryl alkanin in beagle dogs plasma after oral administration of A. euchroma extract.

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UPLC-MS/MS法同时测定比格犬血浆中常山紫草中三种萘醌的含量及其在药代动力学研究中的应用

建立了一种快速、简便、高效的超高效液相色谱-电喷雾-串联质谱(UPLC-ESI-MS/MS)同时测定比格犬血浆中紫草素、异丁基紫草素、β、β α -二甲基丙烯基烷醇的方法，并以紫草素为内标进行了评价。电喷雾电离源工作在负电离模式下。采用多反应监测(MRM)方法，分别定量分析了紫草素m/z 287.0→217.9、异丁基紫草素m/z 357.0→268.9、β、β′-二甲基丙烯基烷素m/z 370.0→270.1和紫草素m/z 177.0→89.0。对该方法的选择性、线性、定量下限、精密度、准确度、回收率、基质效应和稳定性进行了验证。所有验证参数均符合法规指导方针的验收标准。本方法成功地应用于紫草素、异丁基紫草素、β、β -二甲基丙烯基烷素在beagle犬口服紫草提取物后血浆中的药动学研究。

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来源期刊

Acta Chromatographica 化学-分析化学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Acta Chromatographica Open Access Acta Chromatographica publishes peer-reviewed scientific articles on every field of chromatography, including theory of chromatography; progress in synthesis and characterization of new stationary phases; chromatography of organic, inorganic and complex compounds; enantioseparation and chromatography of chiral compounds; applications of chromatography in biology, pharmacy, medicine, and food analysis; environmental applications of chromatography; analytical and physico-chemical aspects of sample preparation for chromatography; hyphenated and combined techniques; chemometrics and its applications in separation science.