Molecular detection of caspase 3, 8, 9 genes and ADIPOR1 (rs2275738) polymorphism in colorectal cancer

IF 3.674 4区工程技术 Q1 Engineering

Applied Nanoscience Pub Date : 2023-06-01 DOI:10.1007/s13204-023-02879-2

Zainab Nizar Jawad

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引用次数: 0

Abstract

The main type of cancer-related world casualties is colorectal cancer (CRC). Colorectal cancer occurs due to many important factors, including genes. These genes are programmed death genes such as caspase 3, 8, 9 and variants in receptor of adiponectin 1 (ADIPOR1) that are related to ‘CRC’ risk. A study was conducted on the high and low gene expression of caspase 3, 8, and 9 genes and their association with the risk of rectal cancer, as well as the association of genotypes in adiponectin receptor 1 (ADIPOR1) to indicate the extent to which these genotypes are related to colorectal cancer compared with the control group. This study was conducted from 2021 to 2022 on 100 patients with colorectal cancer after being medically diagnosed and compared them with 100 healthy individuals. Overall mRNA for all blood samples were taken out and cDNA was produced, use of SYBR Green mix of PCR master and actual gene expression method, quantifying the transcript level of target genes. The polymorphism of the genotypes of ADIPOR1 (rs2275738) was studied using the PCR–RFLP method. Experiments revealed the mRNA expressions of caspases 9 and 3 in patients with lower CRC compared to healthy control patients and controls. Nonetheless, ADIPOR1 (rs2275738) “CC + CT” genotype in comparison to the “TT” genotype was more repeatedly found in CRC cases than in controls. Smoking, body mass index, and family history of colorectal cancer were higher in the CRC group when compared with the control group.

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结直肠癌caspase 3、8、9基因及ADIPOR1 (rs2275738)多态性的分子检测

世界上与癌症相关的主要伤亡类型是结直肠癌(CRC)。结直肠癌的发生有很多重要的因素，包括基因。这些基因是程序性死亡基因，如caspase 3、8、9和脂联素1受体(ADIPOR1)变异，它们与“结直肠癌”风险相关。通过研究caspase 3、8、9基因的高、低表达及其与直肠癌风险的相关性，以及脂联素受体1 (ADIPOR1)基因型的相关性，与对照组比较这些基因型与结直肠癌的相关程度。该研究于2021年至2022年对100名经医学诊断的结直肠癌患者进行了研究，并将其与100名健康人进行了比较。提取所有血样的总mRNA并生成cDNA，采用PCR母材和实际基因表达法的SYBR Green混合，定量目的基因的转录水平。采用PCR-RFLP方法对ADIPOR1 (rs2275738)基因型多态性进行了研究。实验显示，与健康对照组和对照组相比，低结直肠癌患者中caspases 9和caspases 3的mRNA表达。尽管如此，与“TT”基因型相比，ADIPOR1 (rs2275738)“CC + CT”基因型在CRC病例中比在对照组中更常被发现。与对照组相比，结直肠癌组的吸烟、体重指数和结直肠癌家族史均较高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Nanoscience Materials Science-Materials Science (miscellaneous)

CiteScore

7.10

自引率

0.00%

发文量

430

期刊介绍： Applied Nanoscience is a hybrid journal that publishes original articles about state of the art nanoscience and the application of emerging nanotechnologies to areas fundamental to building technologically advanced and sustainable civilization, including areas as diverse as water science, advanced materials, energy, electronics, environmental science and medicine. The journal accepts original and review articles as well as book reviews for publication. All the manuscripts are single-blind peer-reviewed for scientific quality and acceptance.