Semen collection using electroejaculation and sperm parameters in pacas (Cuniculus paca, Linnaeus, 1766)

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia Pub Date : 2023-07-01 DOI:10.1590/1678-4162-12799

A.L. Faino, P. Santos, I. O. Farikoski, F. Souza, V. Ribeiro

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引用次数: 0

Abstract

ABSTRACT Pacas (Cuniculus paca) are highly hunted animals because of the flavor of their meat, and commercial breeding is recommended. However, this species has a relatively low reproductive rate. This study aimed to collect semen from pacas through electroejaculation and obtain the sperm parameters of this species for the first time. Seven male pacas were used, submitted to an anesthetic protocol before stimulation by an electroejaculator appropriate for the species. The stimulus protocol was performed in three series: series I, 10 stimuli with 1 and 2 V; series II, 10 stimuli with 3 and 4 V; and series III, 10 stimuli with 5 V and interval between series of 2 s. The collected material was evaluated for color, volume, motility, vigor, and concentration. The sperm parameters collected showed a mean volume of 0.43±0.33 mL, concentration of 45.5±42.44×106 sperm/mL, motility of 33.33±32.14%, and mean vigor of 2.6±1.15. In this study, the anesthetic protocol did not seem to favor semen collection by electroejaculation in the pacas. The electrical stimulation protocol was able to stimulate all animals in the study; however, there were few samples with sperm cells and a low rate of motility and vigor in most ejaculates.

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使用电射精和pacas中精子参数采集精液（Cuniculus paca，Linnaeus，1766）

骆马(学名:Cuniculus paca)因其肉质鲜美而被大量捕杀，因此建议进行商业养殖。然而，该物种的繁殖率相对较低。本研究旨在通过电射精法采集羊驼精液，首次获得羊驼的精子参数。研究人员使用了7只雄性pacas，在使用适合该物种的电射精器进行刺激之前，将它们置于麻醉状态。刺激方案分为三个系列:系列1,10个刺激，1和2 V;系列II, 10个3 V和4 V刺激;第三组为5 V的10个刺激，每组间隔2 s。收集的材料的颜色，体积，运动性，活力和浓度进行评估。精子平均体积为0.43±0.33 mL，精子浓度为45.5±42.44×106 /mL，活力为33.33±32.14%，活力为2.6±1.15。在这项研究中，麻醉方案似乎不赞成通过电射精在pacas收集精液。电刺激方案能够刺激研究中的所有动物;然而，有精子细胞的样本很少，大多数射精的运动性和活力都很低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 农林科学-兽医学

CiteScore

0.80

自引率

25.00%

发文量

111

审稿时长

9-18 weeks

期刊介绍： Publica artigos originais de pesquisa sobre temas de medicina veterinária, zootecnia, tecnologia e inspeção de produtos de origem animal e áreas afins relacionadas com a produção animal. Atualmente a revista mantém 628 permutas (419 internacionais e 209 nacionais), sendo um verdadeiro suporte para o recebimento de periódicos pela Biblioteca da Escola. A partir de 1999, a Escola de Veterinária delegou à FEP MVZ Editora o encargo do gerenciamento e edição de todas suas publicações, inclusive do Arquivo, ficando somente com o apoio logístico (instalações, equipamentos, pessoal etc.). O apoio financeiro é exercido pelo CNPq/FINEP e pela própria FEP MVZ.