Kick starting method development for hemp analysis using cannabis as equivalent material

Q4 Medicine
Kar-Weng Chan
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Abstract

Cannabis (or marijuana) is detrimental to humans because of its psychoactive effects. The plant materials of this genus have however been credited with its vast nutritional values. As its benefits outweigh harmful effects, some countries have legalized the use of fiber-type cannabis – a plant product called hemp. Hemp is deemed to contain delta-9-tetrahydrocannabinol (THC) below the legal limit (e.g. <0.3% THC) and thus less psychoactive. It is safe to use hemp if it contains no or little THC. So the enforcement bodies must check its level to assure safety prior to sales. Notwithstanding this, hemp cultivation as well as consumption is not lawful in Malaysia. But recently, federal government has shown interest in growing and commercializing hemp. In this regard, an analytical method for identifying hemp must be developed. In the absence of hemp, a liquid chromatographic method was developed using cannabis plants as equivalent materials to mimic the target plant matrix. The validation procedure demonstrated that the method was able to achieve good selectivity with a detection limit =0.0005 mg/mL (or 0.008%) THC. It was precise (imprecision <3%) and accurate (recovery =98.76%) to quantify THC. The detector response was linear up to 0.05 mg/mL (0.8%) THC. This method is cheap and simple for routine application.
以大麻为等效材料的大麻分析启动方法的发展
大麻(或大麻)对人类是有害的,因为它的精神作用。然而,这个属的植物材料被认为具有巨大的营养价值。由于其利大于弊,一些国家已经将纤维型大麻——一种被称为大麻的植物产品的使用合法化。大麻被认为含有低于法定限制的δ -9-四氢大麻酚(THC)(例如<0.3% THC),因此精神活性较低。如果大麻中不含THC或THC含量很少,使用它是安全的。因此,执法机构必须在销售前检查其水平,以确保安全。尽管如此,大麻种植和消费在马来西亚是不合法的。但最近,联邦政府对大麻的种植和商业化表现出了兴趣。在这方面,必须开发一种鉴别大麻的分析方法。在没有大麻的情况下,开发了一种液相色谱方法,使用大麻植物作为等效材料来模拟目标植物基质。结果表明,该方法具有良好的选择性,检出限为0.0005 mg/mL(或0.008%)THC。定量四氢大麻酚精密度(不精密度<3%)、准确度(回收率=98.76%)高。当THC浓度为0.05 mg/mL(0.8%)时,检测器的响应呈线性。这种方法成本低,易于日常应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Z Zagadnien Nauk Sadowych
Z Zagadnien Nauk Sadowych Medicine-Pathology and Forensic Medicine
CiteScore
0.30
自引率
0.00%
发文量
15
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