A comparison of methods for excluding light from stems to evaluate stem photosynthesis

IF 2.7 3区生物学 Q2 PLANT SCIENCES

Applications in Plant Sciences Pub Date : 2023-09-04 DOI:10.1002/aps3.11542

Nadia A. Valverdi, Camilla Acosta, Gabriella R. Dauber, Gregory R. Goldsmith, Eleinis Ávila-Lovera

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Abstract

Premise

A comparison of methods using different materials to exclude light from stems to prevent stem CO₂ exchange (i.e., photosynthesis), without affecting stem conductance to water vapor, surface temperature, and relative humidity, was conducted on stems of avocado trees in California.

Methods and Results

The experiment featured three materials: aluminum foil, paper-based wrap, and mineral-based paint. We examined stem CO₂ exchange with and without the light exclusion treatments. We also examined stem surface temperature, relative humidity, and photosynthetic active radiation (PAR) under the cover materials. All materials reduced PAR and stem CO₂ exchange. However, aluminum foil reduced stem surface temperature and increased relative humidity.

Conclusions

Methods used to study stem CO₂ exchange through light exclusion have historically relied on methods that may induce experimental artifacts. Among the methods tested here, mineral-based paint effectively reduced PAR without affecting stem surface temperature and relative humidity around the stem.

Abstract Image

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叶片叶片光合作用测定方法的比较

在加利福尼亚的牛油果树的茎上进行了一项比较，使用不同的材料来排除茎上的光，以防止茎上的二氧化碳交换(即光合作用)，而不影响茎对水蒸气的电导率、表面温度和相对湿度。实验采用了三种材料:铝箔、纸基包装和矿物基涂料。我们研究了不排除光处理和排除光处理下茎秆的CO2交换。我们还检测了覆盖材料下茎表面温度、相对湿度和光合有效辐射(PAR)。所有材料都降低了PAR并阻止了CO2交换。然而，铝箔降低了茎表面温度，增加了相对湿度。历史上，通过光排斥来研究干CO2交换的方法依赖于可能诱发实验伪影的方法。在这里测试的方法中，矿物基涂料有效地降低了PAR，而不会影响茎干周围的表面温度和相对湿度。

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来源期刊

Applications in Plant Sciences PLANT SCIENCES-

CiteScore

7.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences. APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.