Termiticidal Activity of Methanol Extract of Pegagan (Centella asiatica L. Urban) Toward Coptotermes curvignathus Holmgren

Dina Erliana, C. Banon, A. Avidlyandi, Rahmaga Febriansyah, M. Adfa
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Abstract

Termiticide activity of methanol extract of Centella asiatica L. Urban against Coptotermes curvignathus Holmgren was investigated using the no choice test. The chemical components of methanol extract of C. asiatica were identified by GC-MS. The antitermite activity showed that the percentage of termite mortality increased when the concentrations of extract increased. The concentrations of 25% indicated the highest termite mortality which reached 100% on the sixth day. Profiling GC-MS methanol extract of C. asiatica revealed 85 chemical components. Based on MS spectral data, 56 chemical components were identified which were grouped into fatty acids (27.09%), esters (23.73%), hydrocarbons (16.56%), ethers (8.28%), sesquiterpenes (1.61 %), alcohol (1.55 %), aldehydes (3.50%), monoterpenes (1.08%), diterpenes (0.67%), steroids (0.43%), and ketones (0.31 %). Among these, the presence of palmitic acid, patchouli alcohol, and β-caryophyllene may be responsible for its termiticide activity. Other compounds such as triterpenoids, saponins, flavonoids, tannins, steroids, and glycosides that were reported previously not detected by GC-MS in this study, possibly contributing to its activity.
Pegagan(Centella asiatica L.Urban)甲醇提取物对曲颈黄连的杀虫活性
采用无选择试验法研究了积雪草甲醇提取物对曲颈黄连的灭蚁活性。采用气相色谱-质谱联用技术对积雪草甲醇提取物的化学成分进行了鉴定。抗白蚁活性表明,随着提取物浓度的增加,白蚁死亡率增加。25%的浓度表明白蚁死亡率最高,在第六天达到100%。积雪草的气相色谱-质谱-甲醇提取物分析显示有85种化学成分。根据质谱数据,共鉴定出56种化学成分,分为脂肪酸(27.09%)、酯类(23.73%)、烃类(16.56%)、醚类(8.28%)、倍半萜类(1.61%)、醇类(1.55%)、醛类(3.50%)、单萜类(1.08%)、二萜类(0.67%)、甾类(0.43%)和酮类(0.31%)。其中,棕榈酸、广藿香醇和β-石竹烯的存在可能是其白蚁活性的原因。其他化合物,如三萜类、皂苷、黄酮类、单宁、类固醇和糖苷,这些化合物以前在本研究中未被GC-MS检测到,可能有助于其活性。
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