Multi-target Inhibitory Potency of Active Metabolites Dictates the Antimicrobial Activity of Indigenous Medicinal Plant Leucas biflora: GC-MS Analysis, Biological Evaluations, and Molecular Docking Studies

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-09-15 DOI:10.1080/10496475.2022.2116513

Anwesha Chatterjee, Mayukh Acherjee, B. Das, S. Chakraborty, Harshata Pal

引用次数: 0

Abstract

ABSTRACT The metabolites present in the crude methanolic extract of Leucas biflora were identified and characterized to obtain new leads for compounds with antibacterial effects. The plant extract showed antibacterial effects against both the gram-positive and gram-negative bacteria in disc-diffusion assay. Gas chromatography-mass spectrometry (GC-MS) results revealed nine types of high and low molecular weight chemical entities with varying quantities in the extract. An in silico target-fishing approach identified two compounds with multi-target-directed activities. Two metabolites, 3-Oxo-18-Nor-ent-ros-4-ene-15.alpha.,16-acetonide and 4-Dehydroxy-N-(4,5-methylenedioxy-2-nitrobenzylidene)-tyramine, inhibited two promising bacterial targets, FabH and DNA gyrase B.

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活性代谢产物的多靶点抑制作用决定了当地药用植物双花亮苏铁的抗菌活性：GC-MS分析、生物学评价和分子对接研究

摘要对双花明粗甲醇提取物中存在的代谢产物进行了鉴定和表征，为具有抗菌作用的化合物提供了新的线索。该植物提取物对革兰氏阳性菌和革兰氏阴性菌均有抗菌作用。气相色谱-质谱分析（GC-MS）结果显示，提取物中存在9种不同数量的高分子量和低分子量化学实体。一种电子靶向捕鱼方法鉴定了两种具有多靶向活性的化合物。两种代谢产物，3-Oxo-18-Nor-ent-ros-4-烯-15α，16-丙酮和4-脱氢-N-（4,5-亚甲基二氧基-2-硝基亚苄基）-酪胺，抑制了两个有前景的细菌靶标，FabH和DNA旋转酶B。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.