Enhanced Electrochemical Activity of a Hollow Carbon Sphere/Polyaniline-Based Electrochemical Biosensor for HBV DNA Marker Detection

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2019-03-21 DOI:10.1021/acsbiomaterials.8b01520

Razieh Salimian, Saeed Shahrokhian*, Saba Panahi

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引用次数: 27

Abstract

Herein, we present a novel, simple, and ultrasensitive electrochemical DNA (E-DNA) sensor based on hollow carbon spheres (HCS) decorated with polyaniline (PANI). A thiolated 21-mer oligonucleotide, characteristic of HBV DNA, is immobilized via electrodeposited gold nanoparticles on HCS-PANI. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) are used to characterize the electrochemical properties of the prepared nanocomposite. Scanning electron microscopy is employed to investigate the morphological texture of the fabricated modifier. An enhanced intrinsic signal of PANI is probed to evaluate the biosensing ability of the prepared modifier. The proposed biosensor allows for the detection of the target sequences of HBV DNA at a concentration as low as 10 fM (i.e., 10⁹ DNA copies/mL). In addition, this biosensor demonstrated good capability to differentiate between the perfectly matched target oligonucleotide and three nucleotide-mismatched oligonucleotides. Furthermore, the HCS/PANI-based E-DNA sensor indicates highly sensitive detection of HBV DNA in real samples.

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中空碳球/聚苯胺电化学生物传感器检测HBV DNA标记物的电化学活性增强

在此，我们提出了一种新颖、简单、超灵敏的电化学DNA (E-DNA)传感器，该传感器基于聚苯胺(PANI)修饰的中空碳球(HCS)。通过电沉积金纳米粒子在HCS-PANI上固定化了一种具有HBV DNA特征的硫代21聚寡核苷酸。利用循环伏安法(CV)、差分脉冲伏安法(DPV)和电化学阻抗谱(EIS)对制备的纳米复合材料的电化学性能进行了表征。利用扫描电子显微镜对制备的改性剂的形貌进行了研究。通过检测聚苯胺的增强本征信号来评价所制备的改性剂的生物传感能力。所提出的生物传感器允许在低至10 fM(即109个DNA拷贝/mL)的浓度下检测HBV DNA的目标序列。此外，该生物传感器表现出良好的区分完全匹配的目标寡核苷酸和三个核苷酸不匹配的寡核苷酸的能力。此外，基于HCS/ pani的E-DNA传感器在真实样品中显示出高灵敏度的HBV DNA检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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