The alarming coincidence of toxin genes with staphylococcal cassette Chromosome mec (SCCmec) in clinical MRSA isolates

IF 4.4 2区生物学 Q1 Agricultural and Biological Sciences

Saudi Journal of Biological Sciences Pub Date : 2025-01-01 Epub Date: 2022-02-18 DOI:10.1016/j.sjbs.2022.02.026

Christiana Rezk Bottros Youssef, Ashraf Ahmed Kadry, Amira Mohammed El-Ganiny

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Abstract

Staphylococcus aureus is an opportunistic pathogen causing infections with high morbidity and mortality. MRSA isolates which harbor Staphylococcal Cassette Chromosome mec (SCCmec) and express various types of toxins are the most serious strains. Type of SCCmec and common toxin genes (TGs) among MRSA isolates were investigated in the current study. SCCmec and TGs were detected by multiplex-PCR. Possible correlations between SCCmec and TGs were elucidated statistically using IBM-SPSS software. Staphylococcus-enterotoxins (SEs) were sequenced to investigate their genetic relatedness using Sanger-sequencing, and MEGAX software.

MRSA were detected in 124 isolates, 67 of MRSA isolates (54 %) were harboring a single SCCmec with 40, 21, 5 and 1 isolate for SCCmec II, SCCmec III, SCCmec IV, and SCCmec V, respectively. Furthermore, Four isolates (3.2 %) had both SCCmec II and SCCmec IV. The highest incidence of TGs was recorded for sea (40.3%) and etb (34.6%) genes. Statistically, moderate correlation between toxins and SCCmec type and between presence of toxin genes and severity of infections were detected. All SEs sequences were correlated, sea and see genes were more conserved. Isolates from serious cases revealed different degrees of nucleotide and amino acid substitution among seb, sec, and tsst genes, which might contribute to their increased virulence. This study improves our understanding of S. aureus toxin profile in relation to SCCmec type, and highlight their possible roles in virulence.

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临床MRSA分离株中毒素基因与金黄色葡萄球菌盒染色体mec（SCCmec）惊人的一致性

金黄色葡萄球菌是一种致病菌，具有很高的发病率和死亡率。含有葡萄球菌盒染色体mec （SCCmec）并表达多种毒素的MRSA分离株是最严重的菌株。本研究对MRSA分离株的SCCmec类型和常见毒素基因（TGs）进行了研究。多重pcr检测SCCmec和TGs。采用IBM-SPSS软件对SCCmec与tg之间可能存在的相关性进行统计学分析。采用Sanger-sequencing和MEGAX软件对葡萄球菌-肠毒素（Staphylococcus-enterotoxins, SEs）进行遗传亲缘性测序。共检出124株MRSA，其中67株（54%）为单一SCCmec，其中SCCmec II、SCCmec III、SCCmec IV和SCCmec V分别为40株、21株、5株和1株。4株（3.2%）同时携带SCCmec II和SCCmec IV基因，其中sea（40.3%）和etb（34.6%）基因的TGs发生率最高。统计上，毒素与SCCmec类型、毒素基因存在与感染严重程度之间存在中度相关性。所有se序列均具有相关性，sea和see基因保守性较强。严重病例分离的seb、sec和tsst基因存在不同程度的核苷酸和氨基酸取代，这可能是其毒力增强的原因。本研究提高了我们对金黄色葡萄球菌毒素谱与SCCmec型的理解，并强调了它们在毒力中的可能作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Saudi Journal of Biological Sciences BIOLOGY-

CiteScore

9.30

自引率

4.50%

发文量

551

审稿时长

34 days

期刊介绍： Saudi Journal of Biological Sciences is an English language, peer-reviewed scholarly publication in the area of biological sciences. Saudi Journal of Biological Sciences publishes original papers, reviews and short communications on, but not limited to: • Biology, Ecology and Ecosystems, Environmental and Biodiversity • Conservation • Microbiology • Physiology • Genetics and Epidemiology Saudi Journal of Biological Sciences is the official publication of the Saudi Society for Biological Sciences and is published by King Saud University in collaboration with Elsevier and is edited by an international group of eminent researchers.