Kathleen L. Miller , Izaac Sit , Yi Xiang , Jerry Wu , Jacob Pustelnik , Min Tang , Wisarut Kiratitanaporn , Vicki Grassian , Shaochen Chen
{"title":"Evaluation of CuO nanoparticle toxicity on 3D bioprinted human iPSC-derived cardiac tissues","authors":"Kathleen L. Miller , Izaac Sit , Yi Xiang , Jerry Wu , Jacob Pustelnik , Min Tang , Wisarut Kiratitanaporn , Vicki Grassian , Shaochen Chen","doi":"10.1016/j.bprint.2023.e00284","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Particulate matter has been identified as a significant environmental threat to </span>human health<span><span>. As one of its components, copper oxide nanoparticles<span> (CuO NP) have been found highly potent in cytotoxicity. However, the elucidation of its mechanism is still limited. This study investigated the toxicity of CuO NP toward a cardiac tissue. To better recapitulate the species-specific tissue phenotype and toxin response, we developed a human </span></span>induced pluripotent stem cells<span> (iPSC)-derived cardiac micro-tissue. With the precise deposition of the cell and scaffold material enabled by rapid 3D bioprinting, the cardiac micro-tissue showed a mature phenotype and was incorporated with a force gauge to enable contraction measurement. We discovered an LD</span></span></span><sub>50</sub><span><span> of 7.176 g/mL from the CuO NP treatment outcome of the micro-tissue with a downward trend in tissue force as toxicity increased. We also identified mitochondrial damage and activation of extrinsic </span>apoptosis as a significant pathway to mediate the tissue toxicity.</span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000271","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
引用次数: 0
Abstract
Particulate matter has been identified as a significant environmental threat to human health. As one of its components, copper oxide nanoparticles (CuO NP) have been found highly potent in cytotoxicity. However, the elucidation of its mechanism is still limited. This study investigated the toxicity of CuO NP toward a cardiac tissue. To better recapitulate the species-specific tissue phenotype and toxin response, we developed a human induced pluripotent stem cells (iPSC)-derived cardiac micro-tissue. With the precise deposition of the cell and scaffold material enabled by rapid 3D bioprinting, the cardiac micro-tissue showed a mature phenotype and was incorporated with a force gauge to enable contraction measurement. We discovered an LD50 of 7.176 g/mL from the CuO NP treatment outcome of the micro-tissue with a downward trend in tissue force as toxicity increased. We also identified mitochondrial damage and activation of extrinsic apoptosis as a significant pathway to mediate the tissue toxicity.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.