Ying Betty Li , Caroline Sodja , Marina Rukhlova , Jordan Nhan , Joshua J.A. Poole , Harry Allen , Selam Yimer , Ewa Baumann , Erin Bedford , Hannah Prazak , Will J. Costain , Sangeeta Murugkar , Jean-Philippe St-Pierre , Leila Mostaço-Guidolin , Anna Jezierski
{"title":"Angiogenesis driven extracellular matrix remodeling of 3D bioprinted vascular networks","authors":"Ying Betty Li , Caroline Sodja , Marina Rukhlova , Jordan Nhan , Joshua J.A. Poole , Harry Allen , Selam Yimer , Ewa Baumann , Erin Bedford , Hannah Prazak , Will J. Costain , Sangeeta Murugkar , Jean-Philippe St-Pierre , Leila Mostaço-Guidolin , Anna Jezierski","doi":"10.1016/j.bprint.2023.e00258","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>Angiogenesis plays a </span>pivotal role in development and tissue growth, as well as in pathological conditions such as cancer. Being able to understand the basic mechanisms involved in the </span>vascularization of tissues and angiogenic network formation provides a window to advance the development of </span><em>in vitro</em><span><span><span><span> tissue models and enhance tissue engineering applications. In this study, we leveraged a novel microfluidic-based three dimensional (3D) bioprinting technology and alginate-collagen type I (AGC) bioink, to develop a 3D bioprinting strategy to enable the </span>biofabrication of complex angiogenic networks within the 3D structure. These networks were comprised of </span>simian vacuolating virus<span> 40 (SV40) transformed adult rat brain endothelial cell (SV-ARBEC)-laden hydrogel rings. With </span></span>mechanical properties<span><span><span> relevant for vascular tissue<span> engineering applications, these bioprinted constructs formed spontaneous vascular networks<span><span>, reminiscent of anisotropic tissue-like structures, while retaining high </span>cellular viability. The vascular network formation was accompanied by </span></span></span>extracellular matrix<span><span> (ECM) remodeling, confirming sequential SV-ARBEC mediated collagen type I fiber deposition and reorganization. </span>Treatment<span> with broad spectrum matrix metalloproteinase (MMP) inhibitor supressed SV-ARBEC angiogenic sprouting<span>, highlighting requirements of ECM remodeling in angiogenic network formation. This novel 3D microfluidic bioprinting technology and biocompatible AGC hydrogel fiber rings supported robust SV-ARBEC angiogenesis and corresponding ECM remodeling, allowing us to present a strategy suitable to advancing applications in vascular research and supporting the further development of disease models, novel testing beds for </span></span></span></span>drug discovery and tissue engineering applications.</span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
引用次数: 0
Abstract
Angiogenesis plays a pivotal role in development and tissue growth, as well as in pathological conditions such as cancer. Being able to understand the basic mechanisms involved in the vascularization of tissues and angiogenic network formation provides a window to advance the development of in vitro tissue models and enhance tissue engineering applications. In this study, we leveraged a novel microfluidic-based three dimensional (3D) bioprinting technology and alginate-collagen type I (AGC) bioink, to develop a 3D bioprinting strategy to enable the biofabrication of complex angiogenic networks within the 3D structure. These networks were comprised of simian vacuolating virus 40 (SV40) transformed adult rat brain endothelial cell (SV-ARBEC)-laden hydrogel rings. With mechanical properties relevant for vascular tissue engineering applications, these bioprinted constructs formed spontaneous vascular networks, reminiscent of anisotropic tissue-like structures, while retaining high cellular viability. The vascular network formation was accompanied by extracellular matrix (ECM) remodeling, confirming sequential SV-ARBEC mediated collagen type I fiber deposition and reorganization. Treatment with broad spectrum matrix metalloproteinase (MMP) inhibitor supressed SV-ARBEC angiogenic sprouting, highlighting requirements of ECM remodeling in angiogenic network formation. This novel 3D microfluidic bioprinting technology and biocompatible AGC hydrogel fiber rings supported robust SV-ARBEC angiogenesis and corresponding ECM remodeling, allowing us to present a strategy suitable to advancing applications in vascular research and supporting the further development of disease models, novel testing beds for drug discovery and tissue engineering applications.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.