Genome-Wide Investigation of Knotted Related Homeobox Genes and Identification of a Fiber-Growth-Repressed Knotted Related Homeobox Gene in Ramie

IF 3.3 2区农林科学 Q1 AGRONOMY

Agronomy-Basel Pub Date : 2023-08-31 DOI:10.3390/agronomy13092297

Jianrong Chen, Xueyu Zhang, Fang Liu, Chang Liu, Yinghong Tang, Chunyan Li, Yuan Gong, Xiaojiang Xu, Yanzhou Wang, Toumin Liu

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引用次数: 0

Abstract

The KNOX transcription factor plays crucial roles in regulating fiber growth in plants. Although the genome of ramie, an important fiber crop in China, is available, knotted related homeobox (KNOX) genes have not been systematically explored in this crop. In this study, seven members of the KNOX gene from the ramie genome were identified and assigned to two groups, Class I and II. The intron–exon structure, conserved domain architecture, cis-regulating elements, and expression pattern showed distinct differences among the seven KNOX regulators. One of the genes, Bnt07G011994, encodes an ortholog of Arabidopsis fiber-growth-related KNAT7, and is differentially expressed among barks undergoing different stages of fiber growth. The overexpression of Bnt07G011994 dramatically decreases the fiber number in transgenic Arabidopsis, indicating a negative role played by this gene in modulating fiber growth. Further transcriptome analysis of transgenic Arabidopsis revealed that the overexpression of Bnt07G011994 resulted in an expression change in 14 pectin biosynthesis-/metabolism-related genes. These findings provide a useful foundation for further investigating the function of KNOX genes in ramie, and provide an important insight into the involvement of the ramie KNOX gene in fiber growth.

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苎麻打结相关同源基因的全基因组研究及纤维生长抑制打结相关同源同源基因的鉴定

KNOX转录因子在植物纤维生长调控中起着至关重要的作用。苎麻是中国重要的纤维作物之一，虽然苎麻的基因组已经确定，但对其KNOX基因的系统研究尚不充分。本研究从苎麻基因组中鉴定出7个KNOX基因成员，并将其分为I类和II类两组。在内含子-外显子结构、保守结构域结构、顺式调控元件和表达模式等方面，7个KNOX调控子存在明显差异。其中一个基因Bnt07G011994编码与拟南芥纤维生长相关的KNAT7同源基因，在纤维生长不同阶段的树皮中表达差异。Bnt07G011994过表达显著减少转基因拟南芥的纤维数量，表明该基因在调节纤维生长中起负作用。进一步对转基因拟南芥进行转录组分析发现，Bnt07G011994过表达导致14个果胶生物合成/代谢相关基因的表达变化。这些发现为进一步研究KNOX基因在苎麻中的功能提供了有益的基础，并为苎麻KNOX基因参与纤维生长提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Agronomy-Basel Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

6.20

自引率

13.50%

发文量

2665

审稿时长

20.32 days

期刊介绍： Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.