An Introduction to Indonesian Wild Shiitake

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-08-21 DOI:10.4308/hjb.30.6.1132-1138

I. P. Putra, Mada Triandala, Saipul Sihotang, Lilis Supratman, Rudy Hermawan, O. D. Nurhayat

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引用次数: 0

Abstract

Pegler suggested that shiitake comprises three morphological species: Lentinula edodes (continental and northeast Asia), L. lateritia (tropical Asia and Australasia), and L. novae-zelandiae (New Zealand). The current study reported for the first time the occurrence of L. lateritia (Berk.) Pegler in Indonesia. During a fungus foray in Kerinci (Jambi, Sumatra, Indonesia) in 2022 and 2023 by the Indonesian mushroom hunter community, some basidiomata of Lentinula were obtained. At a glance, our specimens resembled L. edodes. The current study aims to justify the taxonomical position of our specimens based on morphological and molecular data. The fresh basidiomata were used for morphological and molecular analyses. The molecular work was done using ITS 4/5 Primers for phylogenetic analysis of rDNA-ITS region. Morphologically, the uniformly reddish brown, smooth, and glabrous of pileus confirmed our specimens as L. lateritia. In addition, the absence of a range of colors and squamules pileus distinguished L. lateritia BO24628 form L. edodes, while the formation of florets cheilocystidia in L. madagasikarensis was the distinctive character of our specimens. The BLAST result revealed that our specimen has high similarity (99-100%) with L. lateritia and L. edodes as the top hits. The phylogenetic tree (RAxML) nested our specimens in the L. lateria clade and is closely related to one specimen from Papua New Guenia (PNG) (BS 98%). In addition, L. lateritia BO24628 has a sister clade of the specimen from PNG and Australia. Moreover, we provide the herbarium collection of wild L. lateritia in Indonesia.

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印尼野生香菇简介

Pegler认为香菇包括三个形态物种：香菇（亚洲大陆和东北部）、红土香菇（亚洲热带和澳大拉西亚）和泽兰香菇（新西兰）。目前的研究首次报道了红土乳杆菌（Berk.）Pegler在印度尼西亚的出现。2022年和2023年，印尼蘑菇猎人社区在Kerinci（印度尼西亚苏门答腊占碑）进行了一次真菌突袭，获得了一些香菇担子菌。乍一看，我们的标本很像香菇。目前的研究旨在根据形态学和分子数据证明我们标本的分类地位。利用新鲜担子体进行形态和分子分析。利用ITS 4/5引物对rDNA ITS区进行系统发育分析。从形态学上看，菌盖均匀的红棕色、光滑、无毛，证实了我们的标本是红土乳杆菌。此外，由于缺乏一系列颜色和鳞片状菌盖，红土乳杆菌BO24628形成了香菇，而马达加斯加乳杆菌的小花cheilocystia的形成是我们标本的独特特征。BLAST结果显示，我们的标本与红土乳杆菌和香菇有很高的相似性（99-100%）。系统发育树（RAxML）将我们的标本嵌套在L.lateria分支中，并与巴布亚新几内亚（PNG）的一个标本密切相关（BS98%）。此外，红土乳杆菌BO24628有一个来自巴布亚新几内亚和澳大利亚的姐妹分支。此外，我们还提供印度尼西亚野生红土乳杆菌的植物标本馆收藏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.