Changes of Locomotor Activity by Dopamine D2, D3 Agonist Quinpirole in Mice Using Home-cage Monitoring System.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2023-11-30 Epub Date: 2023-05-22 DOI:10.9758/cpn.22.1016
Jeonghyun Park, Eunsoo Moon, Hyun Ju Lim, Kyungwon Kim, Yoo Rha Hong, Jung Hyun Lee
{"title":"Changes of Locomotor Activity by Dopamine D2, D3 Agonist Quinpirole in Mice Using Home-cage Monitoring System.","authors":"Jeonghyun Park,&nbsp;Eunsoo Moon,&nbsp;Hyun Ju Lim,&nbsp;Kyungwon Kim,&nbsp;Yoo Rha Hong,&nbsp;Jung Hyun Lee","doi":"10.9758/cpn.22.1016","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>: As dopamine is closely linked to locomotor activities, animal studies on locomotor activities using dopaminergic agents were widely done. However, most of animal studies were performed for a short period that there is a lack of longitudinal study on the effects of dopaminergic agents on locomotor activities. This study aimed to examine the longterm effect of a dopamine D2, D3 agonist quinpirole on locomotor activities in mice using a home-cage monitoring system.</p><p><strong>Methods: </strong>: The locomotor activities of Institute Cancer Research mice were measured by infrared motion detectors in home-cages under the 12-hour dark and 12-hour light condition for three days after the quinpirole injection. Quinpirole was injected at a concentration of 0.5 mg/kg intraperitoneally in the beginning of the dark phase. The locomotor activities before and after the quinpirole administration were compared by the Wilcoxon signed-rank test and one-way repeated measures ANOVA.</p><p><strong>Results: </strong>: After the quinpirole administration, the 24-hour total locomotor activity did not change (<i>p</i> = 0.169), but activities were significantly increased in the 12-hour dark phase sum (<i>p</i> = 0.013) and decreased in the 12-hour light phase sum (<i>p</i> = 0.009). Significant increases in the activities were observed in the dark-light difference (<i>p</i> = 0.005) and dark-light ratio (<i>p</i> = 0.005) as well.</p><p><strong>Conclusion: </strong>: This study suggests that quinpirole injection entrains the circadian rest-activity rhythm of locomotor activities. Therefore, quinpirole can be a drug that mediates locomotor activity as a dopamine agonist as well as a modulator of the circadian rhythms.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"686-692"},"PeriodicalIF":4.6000,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/56/ac/cpn-21-4-686.PMC10591172.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.9758/cpn.22.1016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/5/22 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: : As dopamine is closely linked to locomotor activities, animal studies on locomotor activities using dopaminergic agents were widely done. However, most of animal studies were performed for a short period that there is a lack of longitudinal study on the effects of dopaminergic agents on locomotor activities. This study aimed to examine the longterm effect of a dopamine D2, D3 agonist quinpirole on locomotor activities in mice using a home-cage monitoring system.

Methods: : The locomotor activities of Institute Cancer Research mice were measured by infrared motion detectors in home-cages under the 12-hour dark and 12-hour light condition for three days after the quinpirole injection. Quinpirole was injected at a concentration of 0.5 mg/kg intraperitoneally in the beginning of the dark phase. The locomotor activities before and after the quinpirole administration were compared by the Wilcoxon signed-rank test and one-way repeated measures ANOVA.

Results: : After the quinpirole administration, the 24-hour total locomotor activity did not change (p = 0.169), but activities were significantly increased in the 12-hour dark phase sum (p = 0.013) and decreased in the 12-hour light phase sum (p = 0.009). Significant increases in the activities were observed in the dark-light difference (p = 0.005) and dark-light ratio (p = 0.005) as well.

Conclusion: : This study suggests that quinpirole injection entrains the circadian rest-activity rhythm of locomotor activities. Therefore, quinpirole can be a drug that mediates locomotor activity as a dopamine agonist as well as a modulator of the circadian rhythms.

Abstract Image

Abstract Image

多巴胺D2、D3激动剂喹匹罗对小鼠运动活动的影响
目的:由于多巴胺与运动活动密切相关,使用多巴胺能药物进行运动活动的动物研究已被广泛开展。然而,大多数动物研究都是在短时间内进行的,缺乏关于多巴胺能药物对运动活动影响的纵向研究。本研究旨在使用家庭笼监测系统检测多巴胺D2、D3激动剂喹吡唑对小鼠运动活动的长期影响。方法:用红外运动探测器测定癌症研究所小鼠注射喹匹罗后3天在家中黑暗12小时、光照12小时条件下的运动活性。在暗期开始时腹膜内注射浓度为0.5mg/kg的喹吡唑。通过Wilcoxon符号秩检验和单向重复测量ANOVA比较喹匹罗给药前后的运动活动。结果:奎匹罗给药后,24小时总运动活性没有变化(p=0.169),但在12小时暗相总和中活性显著增加(p=0.013),在12小时亮相总和中活动减少(p=0.009)。结论:本研究提示喹吡唑注射液具有运动活动的昼夜节律性。因此,喹吡唑可以作为多巴胺激动剂和昼夜节律调节剂,成为一种介导运动活动的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信