Evaluation of SRAP markers efficiency in genetic diversity of Aspergillus flavus from peanut-cropped soils in China

Q3 Agricultural and Biological Sciences

Oil Crop Science Pub Date : 2022-07-01 DOI:10.1016/j.ocsci.2022.08.005

Chushu Zhang , Lifei Zhu , Mian Wang , Yueyi Tang , Haixiang Zhou , Qi Sun , Qiang Yu , Jiancheng Zhang

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Abstract

In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus, we screened out 17 sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs. The size of SRAP fragments ranged from 120 to 2100 bp. Primer pair Me10/Em9 produced the maximum number of polymorphic bands (12 bands), while Me8/Em13 produced the fewest number of polymorphic bands (only 1). Through analysis genetic diversity ability of different sets of primer pairs, the set of 12 primer pairs was selected for SRAP genetic marker of A. flavus. Cluster analysis was performed based on the genetic similarity coefficients, which ranged from 0.53 to 0.89. A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A. flavus samples into 5 main clusters. The results suggested that SRAP marker is a useful molecular technology for the diversity of A. flavus from peanut soils in China.

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中国花生种植土壤黄曲霉遗传多样性SRAP标记效率评价

为了评价SRAP标记对黄曲霉遗传多样性的影响，从150对SRAP引物中筛选出17对能产生清晰、可复制的SRAP条带的引物。SRAP片段的大小在120 ~ 2100 bp之间。Me10/Em9引物产生的多态性条带最多(12条)，Me8/Em13引物产生的多态性条带最少(1条)。通过对不同引物对的遗传多样性能力分析，选择了12对引物对作为黄曲霉SRAP遗传标记。聚类分析遗传相似系数为0.53 ~ 0.89。采用算术平均的非加权对群方法，将黄芪样本分为5个主要聚类。结果表明，SRAP标记是中国花生土壤黄曲霉多样性的一种有用的分子技术。

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