EXPRESSION OF ANTIGEN THOMSEN–FRIEDENREICH RECOGNIZED BY Amaranthus leucocarpus LECTIN IN MCF CELLS STIMULATED WITH LIPOPOLYSACCHARIDE

Abstract

Altered glycosylation is a characteristic of cancer cells, where the overexpression of truncated antigens such as the Thomsen–Friedenreich (TF) antigen (Galβ1, 3GalNAcα1, O-Ser / Thr) The TF antigen is specifically recognized by the Amaranthus leucocarpus (ALL). The MCF-7 breast cancer cell line maintains characteristics similar to differentiated mammary epithelial cells. It has been linked to stimulation of breast cancer cells with lipopolysaccharide, with a higher risk of metastasis in this type of cancer. In this work the expression of TF antigen and moesin in MCF-7 stimulated with LPS at concentration of 20 ng / ml cells was determined, by flow cytometry and fluorescence microscopy. MCF-7 cells express 45% of TF antigen without stimulus with LPS, the expression of the TF antigen decreases with the time of LPS stimulation. Moesin expression increases with LPS stimulation time. A double-positive MCF-7 population for moesin and a TF-antigen of 6.34% were identified by cytometry at 6 hours of LPS stimulation, compared to cells with no stimulus, whose double positivity was 2.86%. Our results suggests that moesin could be Amaranthus leucocarpus receptor in MCF-7 cells.