Correlation between the deletion of phosphatase and tensin homolog gene on chromosome 10, syncytin-1 and the damage of maternal endothelium and trophoblast in preeclampsia placenta

中国综合临床 Pub Date : 2020-03-01 DOI:10.3760/CMA.J.ISSN.1008-6315.2020.02.017

Xiaoshan Fang, Huijin Feng

{"title":"Correlation between the deletion of phosphatase and tensin homolog gene on chromosome 10, syncytin-1 and the damage of maternal endothelium and trophoblast in preeclampsia placenta","authors":"Xiaoshan Fang, Huijin Feng","doi":"10.3760/CMA.J.ISSN.1008-6315.2020.02.017","DOIUrl":null,"url":null,"abstract":"Objective \nTo study the correlation between phosphatase and tensin homolog deleted on chromosome ten(PTEN), Syncytin-1 in preeclamptic placenta and maternal endothelial injury, trophoblast injury. \n \n \nMethods \nFrom February 2015 to December 2017, 46 patients diagnosed as preeclampsia and delivered in Xiaolan Hospital Affiliated to Southern Medical University and 80 pregnant women with normal delivery in the same period were selected.Before delivery, maternal peripheral blood was collected and the content of endothelial injury markers was determined; after delivery, the placenta was retained and the expression of PTEN, Syncytin-1, apoptosis gene and the contents of oxidative stress markers were measured. \n \n \nResults \nThe mRNA expression of PTEN in placental tissue of preeclampsia was significantly higher than that of normal placental tissue(t=32.797, P<0.000), and the mRNA expression of Syncytin-1 was significantly lower than that of normal placental tissue(t=28.506, P<0.001); the content of ET-1 and sFlt-1 in the blood circulation of the maternal body of preeclampsia was significantly higher than thsoe of normal pregnant women(t=39.884, 40.243; all P<0.000) and positively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=0.674, 0.596; P=0.004, 0.007), negatively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=-0.615, -0.637; P<0.009, 0.008); the content of NO, PGI2, VEGF and PLGF were significantly lower than thse of normal pregnant women(t=30.944, 27.404, 32.827, 26.644; all P<0.001) and negatively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=-0.648, -0.592, -0.537, -0.613; P=0.006, 0.009, 0.013, 0.007), positively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=0.632, 0.572, 0.537, 0.654; P<0.010, 0.013, 0.015, 0.009); The contents of NOX4, MDA, 8-OHdG and the mRNA expression of Fas, FasL, Bax, GRP78, CHOP, Caspase-3 in placental tissue of preeclampsia was significantly higher than that of normal placental tissue(t=27.714, 27.704, 26.786, 27.964, 22.622, 25.496, 25.893, 30.837, 28.975; all P<0.001) and positively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=0.703, 0.651, 0.593, 0.628, 0.449, 0.552, 0.612, 0.569, 0.502; P<0.003, 0.007, 0.004, 0.006, 0.016, 0.010, 0.006, 0.012, 0.018), negatively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=-0.615, -0.714, -0.577, -0.548, -0.512, -0.585, -0.619, -0.495, -0.662; P<0.009, 0.001, 0.013, 0.015, 0.018, 0.011, 0.009, 0.023, 0.003); The contents of PrxII, PrxIII, SOD and mRNA expression of Bcl-2 in placental tissue of preeclampsia was significantly lower than that of normal placental tissue(t=26.115, 23.502, 17.194; all P<0.000) and negatively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=-0.713, -0.657, -0.591, -0.642; P<0.001, 0.006, 0.009, 0.007), positively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=0.562, 0.711, 0.682, 0.608, P<0.013, <0.001, 0.002, 0.008). \n \n \nConclusion \nThe high expression of PTEN and the low expression of syncytin-1 in placenta of preeclampsia can aggravate the damage of endothelium and trophoblast. \n \n \nKey words: \nPreeclampsia; Phosphatase and tensin homologue-deleted chromosome ten gene; Syncytin-1; Endothelial injury; Trophoblast injury","PeriodicalId":10365,"journal":{"name":"中国综合临床","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国综合临床","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2020.02.017","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Objective To study the correlation between phosphatase and tensin homolog deleted on chromosome ten(PTEN), Syncytin-1 in preeclamptic placenta and maternal endothelial injury, trophoblast injury. Methods From February 2015 to December 2017, 46 patients diagnosed as preeclampsia and delivered in Xiaolan Hospital Affiliated to Southern Medical University and 80 pregnant women with normal delivery in the same period were selected.Before delivery, maternal peripheral blood was collected and the content of endothelial injury markers was determined; after delivery, the placenta was retained and the expression of PTEN, Syncytin-1, apoptosis gene and the contents of oxidative stress markers were measured. Results The mRNA expression of PTEN in placental tissue of preeclampsia was significantly higher than that of normal placental tissue(t=32.797, P<0.000), and the mRNA expression of Syncytin-1 was significantly lower than that of normal placental tissue(t=28.506, P<0.001); the content of ET-1 and sFlt-1 in the blood circulation of the maternal body of preeclampsia was significantly higher than thsoe of normal pregnant women(t=39.884, 40.243; all P<0.000) and positively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=0.674, 0.596; P=0.004, 0.007), negatively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=-0.615, -0.637; P<0.009, 0.008); the content of NO, PGI2, VEGF and PLGF were significantly lower than thse of normal pregnant women(t=30.944, 27.404, 32.827, 26.644; all P<0.001) and negatively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=-0.648, -0.592, -0.537, -0.613; P=0.006, 0.009, 0.013, 0.007), positively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=0.632, 0.572, 0.537, 0.654; P<0.010, 0.013, 0.015, 0.009); The contents of NOX4, MDA, 8-OHdG and the mRNA expression of Fas, FasL, Bax, GRP78, CHOP, Caspase-3 in placental tissue of preeclampsia was significantly higher than that of normal placental tissue(t=27.714, 27.704, 26.786, 27.964, 22.622, 25.496, 25.893, 30.837, 28.975; all P<0.001) and positively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=0.703, 0.651, 0.593, 0.628, 0.449, 0.552, 0.612, 0.569, 0.502; P<0.003, 0.007, 0.004, 0.006, 0.016, 0.010, 0.006, 0.012, 0.018), negatively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=-0.615, -0.714, -0.577, -0.548, -0.512, -0.585, -0.619, -0.495, -0.662; P<0.009, 0.001, 0.013, 0.015, 0.018, 0.011, 0.009, 0.023, 0.003); The contents of PrxII, PrxIII, SOD and mRNA expression of Bcl-2 in placental tissue of preeclampsia was significantly lower than that of normal placental tissue(t=26.115, 23.502, 17.194; all P<0.000) and negatively correlated with the mRNA expression of PTEN in placenta(correlation coefficient=-0.713, -0.657, -0.591, -0.642; P<0.001, 0.006, 0.009, 0.007), positively correlated with the mRNA expression of Syncytin-1 in placenta(correlation coefficient=0.562, 0.711, 0.682, 0.608, P<0.013, <0.001, 0.002, 0.008). Conclusion The high expression of PTEN and the low expression of syncytin-1 in placenta of preeclampsia can aggravate the damage of endothelium and trophoblast. Key words: Preeclampsia; Phosphatase and tensin homologue-deleted chromosome ten gene; Syncytin-1; Endothelial injury; Trophoblast injury

查看原文本刊更多论文

子痫前期胎盘10号染色体磷酸酶和张力素同源基因、合胞体-1缺失与母体内皮细胞和滋养层损伤的相关性

目的探讨子痫前期胎盘中10号染色体缺失的磷酸酶和张力素同源物（PTEN）、合胞素-1与母体内皮损伤、滋养层损伤的关系。方法选择2015年2月至2017年12月在南方医科大学附属小兰医院分娩的46例先兆子痫患者和同期正常分娩的80例孕妇。分娩前，采集产妇外周血，测定内皮损伤标志物含量；分娩后保留胎盘，检测PTEN、合胞素-1、凋亡基因的表达和氧化应激标志物的含量。结果子痫前期胎盘组织PTEN mRNA表达显著高于正常胎盘组织（t=32.797，P＜0.000），合胞素-1 mRNA表达显著低于正常胎盘组织，t=28.506，P＜0.001）；子痫前期母体血液循环中ET-1和sFlt-1的含量显著高于正常孕妇（t=39.884,40.243；均<0.000），并与胎盘中PTEN的mRNA表达呈正相关（相关系数=0.6740.596；P=0.0004.007），与胎盘中合胞素-1的mRNA表达呈负相关（相关系数=0.615，-0.637；P＜0.009，0.008）；NO、PGI2、VEGF和PLGF含量显著低于正常孕妇（t=30.944、27.404、32.827、26.644；均<0.001），并与胎盘中PTEN的mRNA表达呈负相关（相关系数=0.648、-0.592、-0.537、-0.613；P=0.006、0.009、0.013、0.007），与胎盘中合胞素1的mRNA表达呈正相关（相关系数=0.632，0.572，0.537，0.654；P＜0.010，0.013，0.015，0.009）；NOX4、MDA、8-OHdG含量及Fas、FasL、Bax、GRP78、CHOP、，子痫前期胎盘组织中Caspase-3显著高于正常胎盘组织（t=27.714、27.704、26.786、27.964、22.622、25.496、25.893、30.837、28.975；均P＜0.001），且与胎盘中PTEN的mRNA表达呈正相关（相关系数=0.703、0.651、0.593、0.628、0.449、0.552、0.612、0.569、0.502；P<0.003、0.007、0.004、0.006、0.016、0.010、0.006和0.012、0.018），与胎盘中合胞素-1的mRNA表达呈负相关（相关系数=-0.615、-0.714、-0.577、-0.548、-0.512、-0.585、-0.619、-0.495、-0.662；P<0.0019、0.013、0.015、0.018、0.011、0.009、0.023、0.003）；子痫前期胎盘组织中PrxII、PrxIII、SOD含量和Bcl-2 mRNA表达显著低于正常胎盘组织（t=26.115、23.502、17.194；均P＜0.000），并与胎盘中PTEN mRNA表达呈负相关（相关系数分别为-0.713、-0.657、-0.591、-0.642；P＜0.001、0.006、0.009、0.007），结论子痫前期胎盘中PTEN的高表达和合胞素1的低表达可加重内皮细胞和滋养层的损伤。关键词：子痫前期；磷酸酶和张力蛋白同源物缺失的第十染色体基因；合胞素-1；内皮损伤；滋养细胞损伤

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

中国综合临床

CiteScore

0.10

自引率

0.00%

发文量

16855

期刊介绍： Clinical Medicine of China is an academic journal organized by the Chinese Medical Association (CMA), which mainly publishes original research papers, reviews and commentaries in the field. Clinical Medicine of China is a source journal of Peking University (2000 and 2004 editions), a core journal of Chinese science and technology, an academic journal of RCCSE China Core (Extended Edition), and has been published in Chemical Abstracts of the United States (CA), Abstracts Journal of Russia (AJ), Chinese Core Journals (Selection) Database, Chinese Science and Technology Materials Directory, Wanfang Database, China Academic Journal Database, JST Japan Science and Technology Agency Database (Japanese) (2018) and other databases.