Evaluation of detoxification of aflatoxin-b1 by using Ag nanoparticles of oil extracts user prepared by using some medical herbs

Abstract

Summary Introduction: Herbs or plants are used for a variety of purposes, including nutrition, medicinal and in the beverage industry, beverages, coloring, food preservatives, insect repellants and cosmetics. Methods: Many plants were obtained from local markets and the oil was extracted with hexane at a concentration of 75%. Green silver nanoparticles were prepared. The nanoparticles were characterized using various techniques, including scanning electron microscopy, UV visible spectroscopy, Fourier transform infra-red, energy dispersive spectroscopy, and zeta potential analyzer. Results: The results showed small, smooth spherical nanoparticles, ranging between 57.41–88.00 nm, as well as the distribution of electric charges evenly on the surface of the nanoparticles, which acquired effective agents for nanoparticles against aflatoxin-b1. The effectiveness of green nanoparticles against aflatoxin-b1 by using high-performance liquid chromatography technology detected its concentration. The standard concentration of aflatoxin-b1 was (20) ppb. The results of the activity of plant oil extracts of T-thyme, rosemary-R, mint-M and eucalyptus-E reached (0.104, 1.586, 1.083 and 1.067) ppb, while it appears in the nanoparticle activity of T, R, M and E were as (0.065, 0.226, 0.377 and 0.702) ppb respectively. Conclusion: We concluded that green Ag nanoparticles are efficient in processing or eliminating aflatoxin-b1 and can be produced at very low concentrations compared to the concentrations of plant extracts prepared.