Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet

IF 2.4 Q3 NUTRITION & DIETETICS
Mariana Grancieri , Thaisa Agrizzi Verediano , Cintia Tomaz Sant'Ana , Andressa de Assis , Renata Lopes Toledo , Elvira Gonzalez de Mejia , Hercia Stampini Duarte Martino
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引用次数: 8

Abstract

Background and aim

Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of proteins and peptides with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet.

Methods and results

C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in obese mice (p < 0.05). Furthermore, treatment with DP reduced (p < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA SREBP1 (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA LPL and HDL levels were not changed (p > 0.05). The peptides from DP had in silico high interaction with metalloproteinase-2.

Conclusions

When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.

从鼠尾草籽(鼠尾草)中消化的蛋白质可以防止高脂肪饮食小鼠的肥胖和相关的脂肪组织炎症
背景和目的超重和肥胖与多种健康并发症的发生有关,如糖尿病、癌症和心血管疾病。奇亚籽富含蛋白质和多肽,具有潜在的抗炎和抗氧化作用。本研究旨在评价奇亚籽消化蛋白(DP)对高脂饮食小鼠脂肪生成和脂肪组织炎症的抑制作用。方法和结果采用高脂饲粮加DP (400 mg/kg体/d)饲喂sc57bl /6黑鼠9周。奇亚籽DP降低了肥胖小鼠血浆总胆固醇(- 17.5%)、低密度脂蛋白(- 42.8%)、甘油三酯(- 12.3%)、体脂%(- 26.98%)和腰围(- 5.5%)的水平(p <0.05)。此外,DP治疗降低了(p <0.05)脂肪细胞面积、炎症灶、p-NF-κB p65、PPARγ、mRNA SREBP1(甾醇调节元件结合转录)和TNF-α水平。与未加DP的动物相比,DP也增加了DP处理动物脂肪组织的mRNA脂联素。然而,mRNA LPL和HDL水平没有改变(p >0.05)。从DP中分离出的多肽与金属蛋白酶-2具有较强的相互作用。结论在实验模型中,奇亚籽DP具有抗炎、抗脂肪作用。这些结果表明,从奇亚籽中消化的蛋白质对中心性肥胖及其相关炎症是有效的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PharmaNutrition
PharmaNutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.70
自引率
3.10%
发文量
33
审稿时长
12 days
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