Biotechnological approaches to the preservation and use of bovine ovarian cumulus-oocyte complexes in the system of reproductive technologies

IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY

Agricultural Science and Practice Pub Date : 2020-12-25 DOI:10.15407/AGRISP7.03.054

P. A. Trotskyi, O. Shcherbak, I. M. Lyuta

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引用次数: 1

Abstract

Aim. To study the effect of different concentrations of ethylene glycol and glycerin in equilibration and vitrifi cation solutions on 1) the viability and further development of frozen-thawed bovine ovarian cumulus-oocyte complexes (COCs), cryopreserved by vitrifi cation method, 2) on the effectiveness of inseminating mature oocytes, obtained from them, and 3) on the formation of embryos. Methods. Biotechnological, cryobiological, morphological, cytogenetic, and statistical methods, as well as methods of statistical data processing were used in the research. Results. The results of experimental studies on the effect of different concentrations of ethylene glycol and glycerin in the equilibration solution during cryopreservation of bovine ovarian COCs (n = 502) on their viability and further development after freezing-thawing are presented. We also show the results of the comparative analysis of cryoresistant properties of bovine ovarian COCs (n = 560) using different concentrations of ethylene glycol and glycerin, as cryoprotectants, in the vitrifi cation solution in terms of the viability and maturation of the oocytes, which originated from these COCs, up to metaphase II of meiosis. A comparative analysis of the application of ethylene glycol and glycerol in differ- ent concentrations for the equilibration and vitrifi cation solutions in cryopreserving bovine ovarian COCs (n = 220) demonstrated a relationship between the level of concentration of these cryoprotectants and the number of embryos after in vitro insemination of mature gametes, obtained from these frozen-thawed COCs. Conclusions. It was found that the use of 25 % ethylene glycol and 5 % glycerin in the equilibration solution and 10 % ethylene glycol and 40 % glycerin in the vitrifi cation solution during cryopreservation of bovine ovarian COCs ensures lower toxicity of these solutions and promotes more effi cient (up to 14.3 %) formation and development of embryos after in vitro insemina- tion of mature gametes, obtained from these COCs

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在生殖技术系统中保存和使用牛卵巢卵丘-卵母细胞复合体的生物技术方法

目标研究平衡溶液和玻璃化溶液中不同浓度的乙二醇和甘油对1）通过玻璃化冷冻保存的冻融牛卵巢卵丘-卵母细胞复合物（COCs）的生存能力和进一步发育的影响，2）对从中获得的成熟卵母细胞的受精效果的影响，以及3）对胚胎形成的影响。方法。研究中使用了生物技术、冷冻生物学、形态学、细胞遗传学和统计学方法以及统计数据处理方法。后果介绍了在牛卵巢COCs（n=502）冷冻保存过程中，平衡溶液中不同浓度的乙二醇和甘油对其生存能力和冻融后进一步发育的影响的实验研究结果。我们还展示了在玻璃化阳离子溶液中使用不同浓度的乙二醇和甘油作为冷冻保护剂的绵羊卵巢COCs（n=560）的耐冷冻特性的比较分析结果，即源自这些COCs的卵母细胞的活力和成熟度，直到减数分裂中期II。对不同浓度的乙二醇和甘油在冷冻保存牛卵巢COCs（n=220）中用于平衡和玻璃化处理溶液的应用的比较分析表明，这些冷冻保护剂的浓度水平与成熟配子体外受精后的胚胎安全数量之间存在关系，从这些冷冻解冻的COCs获得。结论。研究发现，在牛卵巢COCs的冷冻保存过程中，在平衡溶液中使用25%乙二醇和5%甘油，在玻璃化溶液中使用10%乙二醇和40%甘油，可以确保这些溶液的低毒性，并促进成熟配子体外受精后胚胎的更有效（高达14.3%）形成和发育，从这些COC获得

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来源期刊

Agricultural Science and Practice AGRICULTURE, MULTIDISCIPLINARY-

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25.00%

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