High-Intensity Ultraviolet-C Irradiation Efficiently Inactivates SARS-CoV-2 Under Typical Cold Chain Temperature

IF 4.1 2区农林科学 Q2 ENVIRONMENTAL SCIENCES

Food and Environmental Virology Pub Date : 2023-03-08 DOI:10.1007/s12560-023-09552-5

Peiru Li, Xianliang Ke, Dongmei Leng, Xian Lin, Wenling Yang, Hainan Zhang, Changqing Tian, Hongbo Xu, Quanjiao Chen

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Abstract

SARS-CoV-2 contaminated items in the cold chain becomes a threat to public health, therefore the effective and safe sterilization method fit for the low temperature is needed. Ultraviolet is an effective sterilization method while its effect on SARS-CoV-2 under low-temperature environment is unclear. In this research, the sterilization effect of high-intensity ultraviolet-C (HIUVC) irradiation against SARS-CoV-2 and Staphylococcus aureus on different carriers at 4 °C and − 20 °C was investigated. The results showed that dose of 15.3 mJ/cm² achieved more than 3 log reduction of SARS-CoV-2 on gauze at 4 °C and − 20 °C. The vulnerability of coronavirus to HIUVC under − 20 °C was not significantly different than those under 4 °C. Four models including Weibull, biphasic, log-linear tail and log linear were used to fit the survival curves of SARS-CoV-2 and Staphylococcus aureus. The biphasic model fitted best with R² ranging from 0.9325 to 0.9878. Moreover, the HIUVC sterilization correlation between SARS-CoV-2 and Staphylococcus aureus was established. This paper provides data support for the employment of HIUVC under low-temperature environment. Also, it provides a method of using Staphylococcus aureus as a marker to evaluate the sterilization effect of cold chain sterilization equipment.

Graphical Abstract

Abstract Image

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高强度紫外线c辐射在典型冷链温度下有效灭活SARS-CoV-2

冷链中被sars - cov污染的物品对公共卫生构成威胁，因此需要适用于低温的有效安全灭菌方法。紫外线是一种有效的杀菌方法，但其在低温环境下对SARS-CoV-2的效果尚不清楚。本研究研究了高强度紫外-C (HIUVC)在4°C和- 20°C条件下对不同载体上的SARS-CoV-2和金黄色葡萄球菌的杀菌效果。结果表明，在4°C和- 20°C条件下，15.3 mJ/cm2的剂量可使纱布上的SARS-CoV-2减少3倍以上。冠状病毒在- 20℃下对HIUVC的易感性与在4℃下无显著差异。采用Weibull、双相、对数线性尾和对数线性4种模型拟合SARS-CoV-2和金黄色葡萄球菌的生存曲线。双相模型拟合最佳，R2范围为0.9325 ~ 0.9878。此外，还建立了SARS-CoV-2与金黄色葡萄球菌的HIUVC杀菌相关性。本文为低温环境下高低温聚氯乙烯的使用提供了数据支持。本发明还提供了一种以金黄色葡萄球菌为标记物评价冷链灭菌设备灭菌效果的方法。图形抽象

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.