The involvement of a PIG3 homolog quinone oxidoreductase gene in maize resistance to insects and fungi demonstrated through transgenic expression in maize callus

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2023-09-01 DOI:10.1016/j.plgene.2023.100429

Patrick F. Dowd, Eric T. Johnson

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引用次数: 0

Abstract

Insect and pathogen damage of maize inhibits sustainable production. Discovery of maize genes coding for products active against both classes of pests would significantly accelerate the rate of development of resistant varieties. A quinone oxidoreductase gene homologous to apoptosis related P53 inducible gene 3 (PIG3) in vertebrates was identified as a pest resistance candidate in a quantitative trait locus region for maize ear rot resistance. The quinone oxidoreductase gene was cloned from a Fusarium resistant inbred of maize and expressed in maize callus. The transformed callus had some significant resistance to the maize pathogen F. graminearum, compared to control transformants, and was often highly resistant to two major caterpillar pests of maize. A band of enhanced reactive oxygen species (ROS) generation in the presence of relevant substrates was noted when protein extracts from the transgenic callus compared to those from control callus were separated by polyacrylamide gel electrophoresis. Thus, presence or introduction of an optimally functional form of this gene should lead to enhanced resistance of maize and other crops to major insect and fungal pests.

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PIG3同源物醌氧化还原酶基因在玉米愈伤组织中的转基因表达表明其参与玉米对昆虫和真菌的抗性

玉米的昆虫和病原体损害抑制了可持续生产。发现编码对这两类害虫都有活性的产品的玉米基因将显著加快抗性品种的发展速度。在玉米穗腐病抗性的数量性状基因座区，鉴定了一个与脊椎动物细胞凋亡相关的P53诱导基因3（PIG3）同源的醌氧化还原酶基因作为害虫抗性候选基因。从玉米抗镰刀菌自交系中克隆了醌氧化还原酶基因，并在玉米愈伤组织中表达。与对照转化体相比，转化的愈伤组织对玉米病原体禾谷镰刀菌具有一些显著的抗性，并且通常对玉米的两种主要毛虫具有高度抗性。当通过聚丙烯酰胺凝胶电泳分离来自转基因愈伤组织的蛋白质提取物与来自对照愈伤组织的蛋白提取物相比时，注意到在相关底物存在的情况下活性氧（ROS）产生增强的带。因此，该基因的最佳功能形式的存在或引入应导致玉米和其他作物对主要昆虫和真菌害虫的抗性增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.