Application of microsatellite DNA method in determining the genetic diversity of farmed broodstocks of Penaeus vannamei (Boone, 1931) in hatchery centers of Bushehr Province

Abstract

The aim of this study was a detection of different populations of Pacific white shrimp (Penaeus vannamei Boone, 1931) farmed broodstocks and to determine their genetic indexes in hatchery centers of Bushehr province. DNA was extracted from the muscle tissue of 30 broodstocks of 11 stocks using a commercial kit. The repeated sequences were amplified using ten specific primers by PCR in the Iranian Shrimp Center. The results showed that the number of alleles was 4.5-5.5 in the studied stocks. The most allele frequency was observed in the farmed broad stocks of the third stock in the H2 hatchery center, 5.2 ± 0/359. Also, the average of observed heterozygosity in the farmed broad stocks of the second stock in the H5 hatchery center obtained 0.669 ± 0.152, more than other stocks in all centers. Genetics indexes of the farmed broad stocks of the third stock in the H2 hatchery center were increased compared with other stocks in Bucher province. The inbreeding coefficients of the first stocks in the H1 and H3 hatchery centers were significantly more than others, respectively, 0.595 ± 0.105 and 0.547 ± 0.145. The increased inbreeding coefficients of the farmed broad stocks in these stocks can be referred to due to the small size of the founder population and biased selection programs. However, this amount is acceptable according to the information of the other hatchery centers in Bushehr province and other countries. Moreover, it was concluded that broodstock with higher diversity should be used to increase the genetic indicators in the hatchery centers.