Aromatase Enzyme Activity and Liver Receptor Homolog-1 Levels in Gestational Diabetes Mellitus

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Enzyme Inhibition Pub Date : 2022-11-03 DOI:10.2174/1573408019666221103145729

N. Bayraktar, Hasip Tas, M. Bayraktar, I. Koyuncu, I. Sari, H. Uyanıkoğlu

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引用次数: 0

Abstract

Gestational diabetes mellitus (GDM) is one of the prediabetes conditions in which high blood sugar levels and body weight increase during pregnancy. The underlying molecular and biochemical mechanisms of GDM are poorly defined. Aromatase enzyme activity is responsible for the conversion of androgens to estrogens and has a share in the regulation of body fat distribution and liver receptor homolog-1 (LRH-1), which plays a critical role in cholesterol transport, acid homeostasis, and steroidogenesis in GDM patients. This study aims to determine the levels of aromatase enzyme and LRH-1 in GDM patients and to investigate the relationship between the levels of aromatase enzyme and LRH-1 and the levels of insulin, HbA 1c and total cholesterol. This prospective cross-sectional study was conducted over eleven months (September 2020 to July 2021). The study population was selected at Harran University Teaching and Research Hospital. The study included 32 GDM patients and 32 healthy pregnants. The automated assay measured serum fasting blood glucose, HbA1c, and insulin levels (AVIDA 1800 Chemistry System; Siemens). Aromatase enzyme activity and LRH-1 levels were determined by using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Aromatase activity decreased in GDM patients while LRH1 increased. Significant differences in means levels of fasting blood glucose (p=0.11), insulin (p= 0.001) and HbA1c (p= 0.001) between the patients and control groups. There was a significant negative correlation between the levels of aromatase and insulin (r=-370, p =0.037). In addition, a positive significant correlation coefficient (r=0.645, p=0.001) was found between HbA1c and total cholesterol among the patients' group. Our findings indicate that there is a negative relationship between aromatase activity and insulin levels. Aromatase and LRH 1 may play a role in the pathogenesis of GDM, and the use of LRH-1 agonists in treating the disease may be considered an alternative treatment in the future. However, additional studies are required to reveal the possible functions of these two proteins in GDM with their mechanisms.

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妊娠期糖尿病的芳香酶活性和肝脏受体同源物-1水平

妊娠期糖尿病(Gestational diabetes mellitus, GDM)是一种糖尿病前期疾病，在妊娠期间出现高血糖和体重增加。GDM的潜在分子和生化机制尚不明确。芳香酶活性负责雄激素向雌激素的转化，并参与调节体脂分布和肝脏受体同源物-1 (LRH-1)，而LRH-1在GDM患者的胆固醇转运、酸稳态和类固醇生成中起关键作用。本研究旨在测定GDM患者芳香化酶和LRH-1水平，探讨芳香化酶和LRH-1水平与胰岛素、HbA 1c、总胆固醇水平的关系。这项前瞻性横断面研究进行了11个月(2020年9月至2021年7月)。研究人群选自哈兰大学教学与研究医院。该研究包括32名GDM患者和32名健康孕妇。自动测定血清空腹血糖、糖化血红蛋白和胰岛素水平(AVIDA 1800化学系统;西门子)。采用商用酶联免疫吸附试验(ELISA)试剂盒检测芳香酶活性和LRH-1水平。GDM患者芳香酶活性降低，LRH1升高。在患者和对照组之间，空腹血糖(p=0.11)、胰岛素(p= 0.001)和糖化血红蛋白(p= 0.001)的平均水平存在显著差异。芳香化酶水平与胰岛素呈显著负相关(r=-370, p =0.037)。此外，患者组HbA1c与总胆固醇呈显著正相关(r=0.645, p=0.001)。我们的研究结果表明芳香化酶活性与胰岛素水平之间存在负相关关系。芳香化酶和LRH 1可能在GDM的发病机制中发挥作用，使用LRH-1激动剂治疗该疾病可能被认为是未来的一种替代治疗方法。然而，需要进一步的研究来揭示这两种蛋白在GDM中的可能功能及其机制。

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.