Uji Toksisitas Subkronis Ekstrak Kayu Kuning ( Arcangelisia flava Merr) terhadap Hepar dan Ginjal

Emalia Rachmawati, E. Ulfa
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引用次数: 7

Abstract

Kayu kuning ( Arcangelisia flava Merr) merupakan tanaman asli Indonesia yang banyak mengandung alkaloid berberin dan flavonoid. Tanaman ini telah terbukti memiliki aktivitas sebagai antioksidan, antibakteri, antikanker, dan antihiperlipidemia, namun belum diketahui pengaruhnya terhadap hepar dan ginjal sehingga penelitian ini bertujuan mengetahui pengaruh ekstrak A. flava terhadap parameter biokimia hepar, histopatologi hepar, dan histopatologi ginjal tikus. Penelitian dilakukan di Laboratorium Biomedik, Fakultas Farmasi Universitas Jember pada bulan Mei–Agustus 2016. Sebanyak 40 ekor tikus jantan berusia 2–3 bulan dengan bobot 200–300 gram dibagi menjadi 4 kelompok. Kelompok kontrol hanya diberi sodium-carboxymethyl cellulose (CMC-Na) 1%, sedangkan kelompok perlakukan diberi ekstrak A. flava dosis 250, 500, dan 750 mg/kgBB per oral selama 28 hari. Pada hari ke-28, dilakukan penentuan kadar SGOT, SGPT, serta pengamatan histopatologi hepar dan ginjal. Nilai SGPT menunjukkan tidak berbeda bermakna antara kelompok kontrol dan kelompok perlakukan (p<0,05). Hasil pengamatan histopatologi hepar menunjukkan pemberian ekstrak A. flava dosis 250, 500, dan 750 mg/kgBB per oral menyebabkan kongesti pembuluh darah hepar, namun tidak mengakibatkan nekrosis. Pengamatan histopatologi pada ginjal tidak terdapat peradangan, namun terdapat perdarahan sedikit pada glomerulus dan interstitial. Simpulan, pemberian ekstrak A. flava dosis 250, 500, 750 mg/kgBB selama 28 hari tidak mengakibatkan kerusakan hepar dan ginjal. SUBCHRONIC TOXICITY TEST OF YELLOW ROOT EXTRACT ( ARCANGELISIA FLAVA MERR) ON HEPAR AND RENAL Kayu kuning ( Arcangelisia flava Merr) is one of Indonesian herbal plant which contain berberine alkaloid and flavonoids. This plant has been proven as antioxidant, antibacterial, anticancer, antihiperlipidemic and antidiabetes. However, the effect of A. flava extract on hepar and renal has not been studied yet. Accordingly, the objective of this study was to evaluate the effect of A. flava extracts towards chemical parameters of hepar, histopathology of hepar and renal rats. This research was conducted in The Biomedic Laboratory, Faculty of Pharmacy of Universitas Jember on May to August 2016. Forty Wistar male rats aged 2–3 moths, weighed 200–300 grams were divided into four groups. Control groups were only given sodium-carboxymethyl cellulose (CMC-Na) 1% orally while tretment groups were given A. flava extracts at dose 250, 500, and 750 mg/kgBW orally for 28 days. On the day 28, the SGOT, SGPT were measured and histopathology of liver and renal were observed. The result of this research showed that SGPT value of control group and treatment group were not significantly different (p<0.05). Histopathogical evaluation of liver showed that oral administration of A. flava extract at dose 250, 500, and 750 mg/kgBW caused congestion of liver blood vessel, but the liver cell did not experience necrosis. Histopathogical observation on renal showed that there was no inflamation but there was a small bleeding in glomerolus and interstitial. In conclusion, the administration of A. flava extract at doze 250, 500 and 750 mg/kbBW for 28 days do not affect the liver and the renal.
对肝和肾脏进行黄色黄黄提取物(Arcangelisia flava Merr)的亚毒性试验
黄木(Arcangelisia flava Merr)是一种真正的印度尼西亚植物,含有许多浆果生物碱和黄酮类化合物。该植物已被证明具有抗氧化、抗菌、抗癌和抗高脂血症的活性,但其对肝脏和肾脏的影响尚不清楚,因此本研究旨在了解黄曲霉提取物对肝素生物化学、肝素组织病理学和小鼠肾脏组织病理学参数的影响。这项研究于2016年5月至8月在詹伯大学药学院生物医学实验室进行。多达40只2-3个月大的雄性大鼠,携带200-300克棉花,分为4组。对照组仅给予1%羧甲基纤维素钠(CMC Na),而治疗组给予黄曲霉提取物,每次口服250、500和750 mg/kg BB,持续28天。第28天进行SGOT、SGPT及肝肾组织病理学检查。SGPT值表明对照组和治疗组之间没有差异(p<0.05)。肝脏组织病理学结果显示,剂量为250、500和750 mg/kgBB的黄曲霉口服提取物会导致肝脏血管充血,但不会导致坏死。肾脏的组织病理学观察没有显示任何生长,但肾小球和间质几乎没有出血。黄曲霉提取物以250500750mg/kg的剂量单次给药28天不会引起肝脏和肾脏损伤。黄根提取物对肝肾的亚慢性毒性试验黄根提取物(ARCANGELISIA FLAVA MERR)是一种含有黄连素生物碱和黄酮类化合物的印尼草本植物。这种植物已被证明具有抗氧化、抗菌、抗癌、抗流感和抗糖尿病的作用。然而,黄曲霉提取物对肝脏和肾脏的影响尚未得到研究。因此,本研究的目的是评估黄曲霉提取物对肝脏化学参数、肝脏和肾大鼠组织病理学的影响。这项研究于2016年5月至8月在詹伯大学药学院生物医学实验室进行。40只Wistar雄性大鼠,年龄2-3个月,体重200-300克,分为四组。对照组仅口服1%羧甲基纤维素钠(CMC-Na),而治疗组口服黄曲霉提取物,剂量分别为250、500和750mg/kgBW,持续28天。在第28天,测量SGOT、SGPT,并观察肝脏和肾脏的组织病理学。本研究结果显示,对照组和治疗组的SGPT值没有显著差异(p<0.05)。肝脏组织病理学评估显示,口服250、500和750mg/kg剂量的黄曲霉提取物会导致肝血管充血,但肝细胞没有坏死。肾组织病理学观察显示肾小球和间质未见炎症,但有少量出血。总之,黄曲霉提取物以250、500和750 mg/kbBW的剂量给药28天不会影响肝脏和肾脏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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