Ntifungal activity and chemical composition of Iranian medicinal herbs against fish pathogenic fungus, Saprolegnia parasitica

IF 0.8 4区农林科学 Q3 FISHERIES

Iranian Journal of Fisheries Sciences Pub Date : 2020-11-01 DOI:10.22092/IJFS.2020.122970

M. Adel, Maryam Dadar, M. Zorriehzahra, R. Elahi, T. Stadtlander

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引用次数: 6

Abstract

This study aimed to identify the chemical composition of essential oil of some Iranian medicinal plants and their antifungal activity against Saprolegnia parasitica in comparison to formalin as positive control under in vitro conditions. The essential oils of Eryngium campestre, Pimpinella affinis, Mentha piperita, Achillea wilhelmsii and Cuminum cyminum were analyzed for their activity by disk diffusion, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) methods. Also, the oil constituents of investigated plants were analyzed by gas chromatography/mass spectrometry (GC/MS). The major constituent of the surveyed essential oils was bornyl acetate (17.9%) in E. campestre, Pregeijene (27.3%)in P. affinis, menthol (48.5%) in M. piperita, 1,8-cineol (25.2%) in A. wilhelmsii, and α-Pinene (29.1%)in C. cyminum. The MIC values for the surveyed essential oil were was 0.5 µg ml-1 for C. cyminum followed by M piperita and E. campestre both having 1 µg ml-1, for P. affinis 2 µg ml-1 and was 4 µg/mL for A. wilhelmsii. The MFC for the mentioned essential oil were with 0.5 µg ml-1 again lowest for C. cyminum, followed by M. piperita and E. campestre with 2 µg ml-1, for P. affinis MFC was 4 µg ml-1 while it was with 8 µg ml-1 highest for essential oils from A. wilhelmsii. The results indicate that the essential oils of C. cyminun, E. campestre and M. piperita could be potential candidates for new plant based antifungal components in aquaculture against S. parasitica.

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伊朗中草药对鱼类病原菌的抑菌活性及化学成分研究

本研究旨在鉴定伊朗部分药用植物挥发油的化学成分，并在体外条件下与阳性对照福尔马林进行比较，确定其对寄生病霉的抑菌活性。采用纸片扩散、最小抑菌浓度(MIC)和最小杀真菌浓度(MFC)法分析了山雀叶、附体小蠊、薄荷、水蛭叶和小茴香挥发油的活性。并采用气相色谱/质谱(GC/MS)分析了所研究植物的油脂成分。所调查精油的主要成分为樟属植物中乙酸龙脑酯(17.9%)、仿木犀草中Pregeijene(27.3%)、花椒属植物中薄荷醇(48.5%)、金莲属植物中1,8-桉叶油醇(25.2%)和香椿属植物中α-蒎烯(29.1%)。所调查精油的MIC值分别为:cyminum为0.5 μ g mL -1，其次是M . piperita和E. campestree，均为1 μ g mL -1, P. affinis为2 μ g mL -1, A. wilhelmsii为4 μ g/mL。香椿精油的MFC最低，为0.5 μ g ml-1，其次是辣椒和野樟精油，为2 μ g ml-1，亲和草精油的MFC为4 μ g ml-1，而野樟精油的MFC最高，为8 μ g ml-1。结果表明，cyminun、E. campestre和M. piperita挥发油可能是水产养殖中抗寄生蜂的新植物性抗真菌成分。

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来源期刊

Iranian Journal of Fisheries Sciences FISHERIES-

CiteScore

2.30

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： 1- Living various species (contains animals and vegetal species) in various aquatic ecosystems. 2- Health and diseases of aquatic species. 3- Determining the stocks and specific time and location for catching and reliable exploitation for sustainable development. 4- Methods of propagation and culture of high value aquatic resources. 5- Aquatic stock assessment and the methods of restocking the high value species and suggestion for rate, areas and the time for releasing fish and other aquatic organisms fries. 6- Pollutant agents and their effects to the environments of aquatic species. 7- Feed and feeding in aquatic organisms. 8- Fish processing and producing new products. 9- The economic and social aspects of fisheries.