Evaluation of ligninolytic activity in spent mushroom substrate from four cultivated mushrooms

IF 20.2 Q1 MATERIALS SCIENCE, PAPER & WOOD
Ana Marión Pérez-Chávez, María Melisa Alberti, Edgardo Albertó
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引用次数: 2

Abstract

Spent mushroom substrate (SMS) is an abundant residue generated by the mushroom industry, which is mostly discarded. In this study, the SMS of four cultivated mushrooms were evaluated as potential sources of ligninolytic enzymes. Pleurotus ostreatus, P. eryngii, P. djamor and Ganoderma lucidum were cultivated in two different substrates to determine growth, mushroom yields and ligninolytic activity of laccase (Lac) and manganese peroxidase (MnP). Maximum activities in the SMS were 3.731 U/g Lac and 0.191 U/g MnP, both for P. eryngii. Colonized mushroom substrates were also analyzed, and higher activities were found in almost all samples. A correlation test between mushroom yields and ligninolytic activities of each culture was made, and we found that high enzymatic activity in the substrate did not result in high mushroom productivity or vice versa. The SMS was a good source of ligninolytic enzymes. Enzymatic activity depended on the mushroom species and its affinity to the substrate, and it was not influenced by the mushroom yields. Production of mushrooms in diverse lignocellulosic substrates is gaining interest, and obtaining ligninolytic enzymes from residual SMS is an excellent alternative in the circular economy concept.

四种栽培蘑菇废菌底物降解木质素活性的评价
菌渣是食用菌生产过程中产生的大量菌渣,大多被丢弃。在本研究中,对四种栽培蘑菇的SMS作为木质素分解酶的潜在来源进行了评估。以平菇(Pleurotus ostreatus)、羊角菇(P. eryngii)、羊角菇(P. djamor)和灵芝(Ganoderma lucidum)为研究材料,测定其生长、蘑菇产量和漆酶(Lac)和锰过氧化物酶(MnP)的木素降解活性。在SMS中的最高活性分别为3.731 U/g Lac和0.191 U/g MnP。对菌落底物也进行了分析,发现几乎所有样品都有较高的活性。结果表明,酵母菌底物酶解活性高并不会导致酵母菌产量高,反之亦然。SMS是木质素分解酶的良好来源。酶活性取决于菌种及其对底物的亲和力,而不受蘑菇产量的影响。在不同的木质纤维素基质上生产蘑菇正在引起人们的兴趣,从剩余的SMS中获得木质素分解酶是循环经济概念中的一个很好的替代方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Bioresources and Bioproducts
Journal of Bioresources and Bioproducts Agricultural and Biological Sciences-Forestry
CiteScore
39.30
自引率
0.00%
发文量
38
审稿时长
12 weeks
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