The surface properties of Shewanella putrefaciens 200 and S. oneidensis MR-1: the effect of pH and terminal electron acceptors

IF 0.9 4区 地球科学 Q4 GEOCHEMISTRY & GEOPHYSICS
Yoko Furukawa, Jason R Dale
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引用次数: 11

Abstract

We investigated the surface characteristics of two strains of Shewanella sp., S. oneidensis MR-1 and S. putrefaciens 200, that were grown under aerobic conditions as well as under anaerobic conditions with trimethylamine oxide (TMAO) as the electron acceptor. The investigation focused on the experimental determination of electrophoretic mobility (EPM) under a range of pH and ionic strength, as well as by subsequent modeling in which Shewanella cells were considered to be soft particles with water- and ion-permeable outermost layers.

The soft layer of p200 is significantly more highly charged (i.e., more negative) than that of MR-1. The effect of electron acceptor on the soft particle characteristics of Shewanella sp. is complex. The fixed charge density, which is a measure of the deionized and deprotonated functional groups in the soft layer polymers, is slightly greater (i.e., more negative) for aerobically grown p200 than for p200 grown with TMAO. On the other hand, the fixed charge density of aerobically grown MR1 is slightly less than that of p200 grown with TMAO. The effect of pH on the soft particle characteristics is also complex, and does not exhibit a clear pH-dependent trend.

The Shewanella surface characteristics were attributed to the nature of the outermost soft layer, the extracellular polymeric substances (EPS) in case of p200 and lypopolysaccharides (LPS) in case of MR1 which generally lacks EPS. The growth conditions (i.e., aerobic vs. anaerobic TMAO) have an influence on the soft layer characteristics of Shewanella sp. cells. Meanwhile, the clear pH dependency of the mechanical and morphological characteristics of EPS and LPS layers, observed in previous studies through atomic force microscopy, adhesion tests and spectroscopies, cannot be corroborated by the electrohydrodynamics-based soft particle characteristics which does not exhibited a clear pH dependency in this study. While the electrohydrodynamics-based soft-particle model is a useful tool in understanding bacteria’s surface properties, it needs to be supplemented with other characterization methods and models (e.g., chemical and micromechanical) in order to comprehensively address all of the surface-related characteristics important in environmental and other aqueous processes.

Abstract Image

腐坏希瓦氏菌200和希瓦氏菌MR-1的表面特性:pH和末端电子受体的影响
研究了以氧化三甲胺(TMAO)为电子受体,在好氧和厌氧条件下培养的希瓦氏菌S. oneidensis MR-1和S. putrefaciens 200的表面特性。该研究的重点是在一定pH值和离子强度下的电泳迁移率(EPM)的实验测定,以及随后的建模,其中希瓦氏菌细胞被认为是具有水和离子渗透的最外层的软颗粒。p200的软层明显比MR-1具有更高的电荷(即更多的负电荷)。电子受体对希瓦氏菌软粒子特性的影响是复杂的。固定电荷密度是衡量软层聚合物中去离子化和去质子化官能团的指标,有氧生长的p200比用TMAO生长的p200略大(即更负)。另一方面,好氧培养的MR1的固定电荷密度略低于TMAO培养的p200。pH对软颗粒特性的影响也很复杂,并没有明显的pH依赖趋势。Shewanella的表面特征归因于最外层软层的性质,p200的胞外聚合物(EPS)和MR1的胞外多糖(LPS),而MR1通常缺乏EPS。生长条件(即好氧与厌氧TMAO)对Shewanella sp.细胞的软层特性有影响。同时,以往研究中通过原子力显微镜、粘附试验和光谱学观察到的EPS和LPS层的力学和形态特征明显的pH依赖性,并不能被基于电流体动力学的软颗粒特征所证实,在本研究中,软颗粒特征并没有表现出明显的pH依赖性。虽然基于电流体动力学的软颗粒模型是理解细菌表面特性的有用工具,但它需要补充其他表征方法和模型(例如,化学和微机械),以便全面解决环境和其他水处理过程中所有重要的表面相关特性。
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来源期刊
Geochemical Transactions
Geochemical Transactions 地学-地球化学与地球物理
CiteScore
3.70
自引率
4.30%
发文量
2
审稿时长
>12 weeks
期刊介绍: Geochemical Transactions publishes high-quality research in all areas of chemistry as it relates to materials and processes occurring in terrestrial and extraterrestrial systems.
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