Development of microsatellite DNA markers for Indian Rat snake (Ptyas mucosa)

Abstract

Thirteen microsatellite markers were developed for one of the vulnerable snake species Indian Rat snake (Ptyas mucosa) in Indian ecosystems that are now subject to much restoration efforts. All loci were polymorphic when tested across 25 individuals. The average number of alleles per locus varied from 4 to 7 (mean 5.2). Observed and expected heterozygosity ranged from 0.76 to 1.00 and 0.59–0.94, respectively and the average polymorphic information content is 0.664 were identified demonstrating the utility of these markers for the investigation of the population genetic structure and diversity of this species.