Proteomic analysis of acute radiation-induced rectal injury in rats

Q1 Health Professions

Radiation Medicine and Protection Pub Date : 2023-09-01 DOI:10.1016/j.radmp.2023.08.003

Yue Zhu , Jun Dai , Xiaopeng Xu , Yi Gao , Weidong Shen , Shuyu Zhang , Pengfei Liu

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引用次数: 0

Abstract

Objective

To comprehensively elucidate overall protein alterations associated with acute radiation-induced rectal injury in rats.

Methods

A rat model of acute radiation-induced rectal injury was established by irradiating rectal segments with a single dose of 17.5 Gy X-rays. These segments were then collected at 7 d and 10 d post-irradiation. Tandem mass tag-based quantitative proteomic analysis was then performed.

Results

65,526 peptides were identified, corresponding to 8,088 proteins. Hematoxylin-eosin staining revealed characteristic epithelial cell degeneration and necrosis, intestinal gland atrophy and dilatation, and interstitial inflammatory cell infiltration. Inflammation was more pronounced in the 10 d irradiation group than in the 7 d irradiation group. Overall, 127 up- and 108 downregulated proteins were identified at 7 d post-irradiation, and 122 up- and 44 downregulated proteins were identified at 10 d post-irradiation. Notably, 17 up- and 6 downregulated proteins were consistently co-expressed at both time points. The expression of three of these proteins was validated via real-time quantitative PCR: polypeptide YY (Pyy), thymidylate synthase (Tyms), and tetraspanin (CD9). Tyms transcript levels were significantly higher in irradiated rectal tissues (P < 0.05). Pyy transcript levels were significantly higher at both time points (P < 0.05). Finally, CD9 mRNA expression was significantly lower in both the 7 d and 10 d irradiation groups (P < 0.05).

Conclusions

The potential targets were found to prevent and treat acute radiation-induced rectal injury in clinical practice.

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急性辐射致大鼠直肠损伤的蛋白质组学分析

目的全面阐明大鼠急性放射性直肠损伤相关蛋白的整体改变。方法采用17.5 Gy单剂量x射线照射大鼠直肠节段，建立急性放射性直肠损伤模型。然后在照射后7 d和10 d收集这些节段。然后进行串联质量标记定量蛋白质组学分析。结果共鉴定出65,526条多肽，对应8088个蛋白。苏木精-伊红染色显示特征性上皮细胞变性坏死，肠腺萎缩扩张，间质炎性细胞浸润。炎症反应在10 d照射组较7 d照射组更为明显。总体而言，在辐照后7天鉴定出127个上调和108个下调蛋白，在辐照后10天鉴定出122个上调和44个下调蛋白。值得注意的是，17个上调蛋白和6个下调蛋白在两个时间点一致共表达。通过实时定量PCR验证了其中三种蛋白的表达:多肽YY (Pyy)，胸腺苷酸合成酶(Tyms)和四跨蛋白(CD9)。辐照直肠组织中Tyms转录水平显著升高(P <0.05)。Pyy转录物水平在两个时间点均显著升高(P <0.05)。最后，CD9 mRNA的表达在7 d和10 d辐照组均显著降低(P <0.05)。结论在临床实践中找到了预防和治疗急性放射性直肠损伤的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Radiation Medicine and Protection Health Professions-Emergency Medical Services

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

103 days