Protective Effect of Melatonin on Nonylphenol-Induced Reproductive and Behavioral Disorders in First-Generation Adult Male Rats

IF 2.7 4区医学 Q2 CLINICAL NEUROLOGY

Behavioural Neurology Pub Date : 2022-04-26 DOI:10.1155/2022/1877761

Mahsa Tavakoli, A. Moghadamnia, F. Pourabdolhossein, M. Asghari, S. Kazemi

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引用次数: 1

Abstract

Methods Pregnant Wistar rats were randomly assigned into five groups: control, NP (25 mg/kg), NP (25 mg/kg)+MLT (10 mg/kg), NP (25 mg/kg)+MLT (20 mg/kg), and MLT (20 mg/kg). The duration of treatment was 21 days from gestation time. Morris water maze was used to assess learning and memory. NP concentrations of serum and testicular tissue were measured by HPLC. Histological analysis of testicular tissues was done by H&E staining. Results Behavioral study showed that NP does not impair learning and memory in first-generation rats. Histomorphometric results showed that NP can significantly reduce the cross-sectional area of the seminiferous tubules and the epithelium, the diameter and number of seminiferous tubules, the thickness of the epithelium, and the number of spermatocytes and spermatogonia compared to other groups. MLT reversed the NP-induced histomorphometric. Also, it changes and increased the activity of superoxide dismutase (SOD), total antioxidant capacity (TAC), and catalase (CAT). The level of malondialdehyde (MDA) significantly decreased in MLT-treated groups compared with the NP group. Conclusion Our finding showed that MLT enhanced the learning process and reduced NP-induced testicular tissue damage through its antioxidants and cytoprotective effects.

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褪黑素对壬基酚诱导的第一代成年雄性大鼠生殖和行为障碍的保护作用

方法将妊娠Wistar大鼠随机分为5组：对照组、NP（25 mg/kg）、NP（25 mg/kg）+MLT（10 mg/kg）、NP（25 mg/kg）+MLT（20 mg/kg）和MLT（20 mg/kg）。治疗时间为妊娠后21天。Morris水迷宫用于评估学习和记忆。用高效液相色谱法测定血清和睾丸组织中NP的浓度。通过H&E染色对睾丸组织进行组织学分析。结果行为学研究表明，NP对第一代大鼠的学习记忆没有损害。组织形态计量学结果显示，与其他组相比，NP可显著减少曲精管和上皮的横截面积、曲精管的直径和数量、上皮的厚度以及精母细胞和精原细胞的数量。MLT逆转了NP诱导的组织形态计量学。此外，它还改变并增加了超氧化物歧化酶（SOD）、总抗氧化能力（TAC）和过氧化氢酶（CAT）的活性。与NP组相比，MLT处理组的丙二醛（MDA）水平显著降低。结论MLT通过其抗氧化剂和细胞保护作用，增强了学习过程，减少了NP诱导的睾丸组织损伤。

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来源期刊

Behavioural Neurology 医学-临床神经学

CiteScore

5.40

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： Behavioural Neurology is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on various diseases and syndromes in behavioural neurology. The aim of the journal is to provide a platform for researchers and clinicians working in various fields of neurology including cognitive neuroscience, neuropsychology and neuropsychiatry. Topics of interest include: ADHD Aphasia Autism Alzheimer’s Disease Behavioural Disorders Dementia Epilepsy Multiple Sclerosis Parkinson’s Disease Psychosis Stroke Traumatic brain injury.