Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma

Q3 Medicine

Biomedical Photonics Pub Date : 2022-02-04 DOI:10.24931/2413-9432-2021-10-4-35-43

A. Rynda, V. Olyushin, D. M. Rostovtsev, Y. Zabrodskaya, G. V. Papayan

{"title":"Fluorescent diagnostics with chlorin e6 in surgery of low-grade glioma","authors":"A. Rynda, V. Olyushin, D. M. Rostovtsev, Y. Zabrodskaya, G. V. Papayan","doi":"10.24931/2413-9432-2021-10-4-35-43","DOIUrl":null,"url":null,"abstract":"Intraoperative fluorescence diagnostics of high-grade gliomas is widely used in neurosurgical practice. This work analyzes the possibilities of fluorescence diagnostics for low-grade gliomas (LGG) using chlorin e6 photosensitizer. The study included patients with newly diagnosed LGG, for whom chlorin e6 was used for intraoperative fluorescence control at a dose of 1 mg/kg. During the operation, the fluorescence intensity of various areas of the putative tumor tissue was analyzed using the RSS Cam – Endo 1.4.313 software. Tissue samples with various degrees of fluorescence intensity were compared with the results of their histopathological analysis (WHO tumor diagnosis, Ki-67 index, P53, VEGF). Fluorescence was detected in more than half of the cases, but in most cases had a focal character and low fluorescence intensity. The fluorescence intensity directly correlated with the data of histopathological examination of tumor tissues (Ki-67 index (p=0.002), expression of P53 (p=0.0015) and VEGF (p=0.001)). The sensitivity of the method for LGG surgery was 72%, the specificity was 56,7%. Intraoperative fluorescence diagnostics with chlorin e6 can be used in LGG surgery, especially to visualize intratumoral areas with a higher degree of anaplasia.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Photonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24931/2413-9432-2021-10-4-35-43","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 3

Abstract

Intraoperative fluorescence diagnostics of high-grade gliomas is widely used in neurosurgical practice. This work analyzes the possibilities of fluorescence diagnostics for low-grade gliomas (LGG) using chlorin e6 photosensitizer. The study included patients with newly diagnosed LGG, for whom chlorin e6 was used for intraoperative fluorescence control at a dose of 1 mg/kg. During the operation, the fluorescence intensity of various areas of the putative tumor tissue was analyzed using the RSS Cam – Endo 1.4.313 software. Tissue samples with various degrees of fluorescence intensity were compared with the results of their histopathological analysis (WHO tumor diagnosis, Ki-67 index, P53, VEGF). Fluorescence was detected in more than half of the cases, but in most cases had a focal character and low fluorescence intensity. The fluorescence intensity directly correlated with the data of histopathological examination of tumor tissues (Ki-67 index (p=0.002), expression of P53 (p=0.0015) and VEGF (p=0.001)). The sensitivity of the method for LGG surgery was 72%, the specificity was 56,7%. Intraoperative fluorescence diagnostics with chlorin e6 can be used in LGG surgery, especially to visualize intratumoral areas with a higher degree of anaplasia.

查看原文本刊更多论文

低级别胶质瘤手术中氯e6荧光诊断

术中荧光诊断高度胶质瘤在神经外科实践中被广泛应用。本文分析了氯e6光敏剂用于低级别胶质瘤(LGG)荧光诊断的可能性。该研究纳入了新诊断的LGG患者，对其使用氯e6以1 mg/kg的剂量进行术中荧光控制。术中应用RSS Cam - Endo 1.4.313软件分析推定肿瘤组织各区域的荧光强度。将不同荧光强度的组织标本与其组织病理学分析结果(WHO肿瘤诊断、Ki-67指数、P53、VEGF)进行比较。荧光在半数以上的病例中检测到，但在大多数病例中具有局灶性和低荧光强度。荧光强度与肿瘤组织病理检查数据(Ki-67指数(p=0.002)、P53表达(p=0.0015)、VEGF表达(p=0.001)直接相关。该方法对LGG手术的敏感性为72%，特异性为56.7%。术中氯e6荧光诊断可用于LGG手术，尤其可用于观察瘤内发育不全程度较高的区域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedical Photonics Medicine-Surgery

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： The main goal of the journal – to promote the development of Russian biomedical photonics and implementation of its advances into medical practice. The primary objectives: - Presentation of up-to-date results of scientific and in research and scientific and practical (clinical and experimental) activity in the field of biomedical photonics. - Development of united Russian media for integration of knowledge and experience of scientists and practitioners in this field. - Distribution of best practices in laser medicine to regions. - Keeping the clinicians informed about new methods and devices for laser medicine - Approval of investigations of Ph.D candidates and applicants.