Bidirectional Communication between Droplet Interface Bilayers Driven by Cell-Free Quorum Sensing Gene Circuits**

IF 3.1 Q2 CHEMISTRY, MULTIDISCIPLINARY
Dr. David T. Gonzales, Surased Suraritdechachai, Dr. Christoph Zechner, Dr. T.-Y. Dora Tang
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引用次数: 0

Abstract

Building synthetic multicellular systems using non-living molecular components is a grand challenge in the field of bottom-up synthetic biology. Towards this goal, a diverse range of chemistries have been developed to provide mechanisms of intercellular communication and methods to assemble multicellular compartments. However, building bottom-up synthetic multicellular systems is still challenging because it requires the integration of intercellular reaction networks with compatible cellular compartment properties. In this study, we encapsulated cell-free expression systems (CFES) expressing two quorum sensing genetic circuits into droplet interface bilayer (DIB) synthetic cells to demonstrate bidirectional communication. We further develop a method of generating custom DIB multicellular structures by acoustic liquid handling to automatically dispense the CFES droplets and show the potential for multiplexing compartmentalized gene circuits for generating heterogeneous populations of cells. Our work provides a step towards building more complex multicellular systems with intercellular communication from the bottom-up to study and experimentally model biological multiscalar processes.

Abstract Image

由无细胞群体感应基因电路驱动的液滴界面双层之间的双向通信
利用非活性分子成分构建合成多细胞系统是自下而上合成生物学领域的一个巨大挑战。为了实现这一目标,各种各样的化学物质已经被开发出来,以提供细胞间通讯的机制和组装多细胞室的方法。然而,构建自下而上的合成多细胞系统仍然具有挑战性,因为它需要将细胞间反应网络与兼容的细胞隔室特性相结合。在这项研究中,我们将表达两个群体感应遗传回路的无细胞表达系统(CFES)封装到液滴界面双层(DIB)合成细胞中,以实现双向通信。我们进一步开发了一种通过声波液体处理产生定制DIB多细胞结构的方法,以自动分配CFES液滴,并显示了用于产生异质细胞群体的多路分隔基因电路的潜力。我们的工作为构建更复杂的多细胞系统提供了一步,从自下而上的细胞间通信到研究和实验模拟生物多标量过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
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