The Morphology, Ultrastructure and Molecular Phylogeny of a New Soil-Dwelling Kinetoplastid Avlakibodo gracilis gen. et sp. nov. (Neobodonida; Kinetoplastea)

IF 1.9 3区生物学 Q4 MICROBIOLOGY

Protist Pub Date : 2022-08-01 DOI:10.1016/j.protis.2022.125885

Artem O. Belyaev , Dmitriy G. Zagumyonnyi , Alexander P. Mylnikov , Denis V. Tikhonenkov

{"title":"The Morphology, Ultrastructure and Molecular Phylogeny of a New Soil-Dwelling Kinetoplastid Avlakibodo gracilis gen. et sp. nov. (Neobodonida; Kinetoplastea)","authors":"Artem O. Belyaev , Dmitriy G. Zagumyonnyi , Alexander P. Mylnikov , Denis V. Tikhonenkov","doi":"10.1016/j.protis.2022.125885","DOIUrl":null,"url":null,"abstract":"<div>Kinetoplastids represent a stockpile of undiscovered protist diversity. Free-living members of this group have been studied less intensively compared to their important parasitic relatives. We have isolated a new soil-dwelling bacteriotrophic kinetoplastid, which is described here as a new genus and new species, Avlakibodo gracilis gen. et sp. nov. Phylogenetic analysis of 18S rRNA genes showed highly supported sister relationship of this protist with the clade uniting Neobodo borokensis, Neobodo curvifilus, Neobodo saliens, Actuariola framvarensis, some Neobodo designis isolates and several environmental sequences, with high statistical support. We have reconstructed the organization of the microtubular cytoskeleton of A. gracilis and determined the origins of the main bands of microtubules. Characteristic ultrastructural features include cytostome associated microtubules (FAS), cytopharynx associated additional microtubules (CMT), microtubular prism (nemadesm) and three microtubular roots (R1, R2 and R3).</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protist","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S143446102200030X","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 2

Abstract

Kinetoplastids represent a stockpile of undiscovered protist diversity. Free-living members of this group have been studied less intensively compared to their important parasitic relatives. We have isolated a new soil-dwelling bacteriotrophic kinetoplastid, which is described here as a new genus and new species, Avlakibodo gracilis gen. et sp. nov. Phylogenetic analysis of 18S rRNA genes showed highly supported sister relationship of this protist with the clade uniting Neobodo borokensis, Neobodo curvifilus, Neobodo saliens, Actuariola framvarensis, some Neobodo designis isolates and several environmental sequences, with high statistical support. We have reconstructed the organization of the microtubular cytoskeleton of A. gracilis and determined the origins of the main bands of microtubules. Characteristic ultrastructural features include cytostome associated microtubules (FAS), cytopharynx associated additional microtubules (CMT), microtubular prism (nemadesm) and three microtubular roots (R1, R2 and R3).

查看原文本刊更多论文

一种新的土栖动质体Avlakibodo gracilis gen. et sp. nov. (Neobodonida)的形态、超微结构和分子系统发育Kinetoplastea)

着丝质体代表了未被发现的原生生物多样性的储备。与它们重要的寄生亲戚相比，对这一群体的自由生活成员的研究不那么深入。我们分离到了一个新的土壤细菌营养动胞体(Avlakibodo gracilis gen. et sp. 11)，本文将其描述为一个新属新种。系统发育分析表明，该原生生物与Neobodo borokensis、Neobodo curvifilus、Neobodo saliens、Actuariola framvarensis、Neobodo designis的一些分离株和一些环境序列具有高度的统计学支持。我们重建了薄叶草微管细胞骨架的组织结构，并确定了微管主要带的来源。典型的超微结构特征包括细胞壁相关微管(FAS)、细胞咽部相关附加微管(CMT)、微管棱镜(nemadesm)和三个微管根(R1、R2和R3)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Protist 生物-微生物学

CiteScore

3.60

自引率

4.00%

发文量

审稿时长

18.7 weeks

期刊介绍： Protist is the international forum for reporting substantial and novel findings in any area of research on protists. The criteria for acceptance of manuscripts are scientific excellence, significance, and interest for a broad readership. Suitable subject areas include: molecular, cell and developmental biology, biochemistry, systematics and phylogeny, and ecology of protists. Both autotrophic and heterotrophic protists as well as parasites are covered. The journal publishes original papers, short historical perspectives and includes a news and views section.