First report of cucurbit chlorotic yellows virus affecting squash and pumpkin in Florida, USA

IF 1.7 Q2 PLANT SCIENCES

Plant Health Progress Pub Date : 2023-02-03 DOI:10.1094/php-01-23-0006-br

Abdul Kader Jailani Amirudeen, Susannah DaSilva, J. Freeman, K. Dey, M. Velez-Climent, John D. McVay, S. Bag, M. Paret

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引用次数: 1

Abstract

A field survey was conducted in Florida during 2020-2021 in squash and pumpkin research fields to identify the viruses associated with leaf symptoms of yellowing, crumpling, and vein yellowing. The symptoms were similar to previously reported whitefly-transmitted viruses such as cucurbit leaf crumple virus (CuLCrV), cucurbit yellow stunting disorder virus (CYSDV), squash vein yellowing virus (SqVYV). Another potential virus of interest was the cucurbit chlorotic yellows virus (CCYV) that was recently reported on watermelon in Florida. Symptomatic leaves were tested by RT-PCR with coat protein (CP) gene-specific (GS) primers for CuLCrV, CYSDV, CCYV, and for SqVYV with a nuclear inclusion protein (NIa) GS primers. Amplification for CuLCrV, CYSDV, and CCYV were detected in squash and pumpkin samples. The CCYV amplicons were further sequenced and compared with available CCYV sequences. The NCBI BLAST analysis revealed similarities of the RdRP (100%), HSP70h (99.75%), and CP (99.75%) to the Shanghai CCYV isolate (RNA KY400636 and RNA2 KY400633). To our knowledge, this is the first report of CCYV on squash and pumpkin in Florida.

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美国佛罗里达州南瓜和南瓜感染葫芦绿黄色病毒的首次报告

2020-2021年，佛罗里达州在南瓜和南瓜研究领域进行了一项实地调查，以确定与叶片发黄、起皱和叶脉发黄症状相关的病毒。症状与先前报道的粉虱传播病毒相似，如葫芦叶皱缩病毒（CuLCrV）、葫芦黄发育迟缓障碍病毒（CYSDV）、南瓜静脉黄变病毒（SqVYV）。另一种令人感兴趣的潜在病毒是最近在佛罗里达州西瓜上报道的葫芦绿黄病毒（CCYV）。用CuLCrV、CYSDV、CCYV的外壳蛋白（CP）基因特异性（GS）引物和核内含蛋白（NIa）GS引物对症状叶片进行RT-PCR检测。在南瓜和南瓜样品中检测到CuLCrV、CYSDV和CCYV的扩增。对CCYV扩增子进行进一步测序，并与可用的CCYV序列进行比较。NCBI BLAST分析显示RdRP（100%）、HSP70h（99.75%）和CP（99.75%。据我们所知，这是CCYV在佛罗里达州首次报道南瓜和南瓜。

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来源期刊

Plant Health Progress Agricultural and Biological Sciences-Horticulture

CiteScore

3.00

自引率

13.00%

发文量

期刊介绍： Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.