Comparison of Berberine and Dexamethasone on Blood and Bronchial Inflammatory Cells of Ovalbumin Sensitized Guinea Pigs

Esculapio Pub Date : 2021-03-31 DOI:10.51273/ESC21.251717

Syeda Tahira Zaidi, R. Kausar, M. Malik, Javeria Sarfraz, Abdullah Shafiq, S. Chiragh

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引用次数: 1

Abstract

Objective: To compare the anti-inflammatory effects of berberine and dexamethasone in ovalbumin sensitized guinea pigs. Methods: This experimental controlled study was conducted in April, 2016 at Postgraduate Medical Institute, Lahore. Twenty- four healthy guinea pigs were selected for study. Six of these were assigned randomly in each group; normal control, ovalbumin (OVA) sensitized, berberine treated and dexamethasone treated groups. Airway inflammation was induced on day 0 and 14 by OVA injections via peritoneal route and by inhalation on 25th, 26th and 27th day in each group excluding the normal control. Berberine (1.8 mg/kg) and dexamethasone (20 mg/kg) were introduced via peritoneal route 30 minutes earlier to each trial in berberine treated and dexamethasone treated groups respectively. Results: Total leukocyte count (TLC) in blood sample of berberine treated group (9990±1346 mm3) and dexamethasone treated group (9054±1432 mm3) was significantly low than OVA sensitized group (14261±3151 mm3). TLC of broncho-alveolar lavage (BAL) fluid in berberine treated group (384±26 mm3) and dexamethasone treated group (306±86) mm3 was significantly low than OVA sensitized group (598±110 mm3). Eosinophil percentage in blood of berberine treated (21.50±3.08) and dexamethasone treated (13.33±5.65) groups were significantly low than OVA sensitized group (30.33±6.74), while eosinophil percentage in BAL fluid was 26.00±6.69 and 21.00±7.46 in berberine treated and dexamethasone treated groups respectively with significant difference from OVA sensitized group value of 40.00±7.79. Conclusion: Berberine and dexamethasone both had reduced TLC and eosinophil percentage in both blood and BAL fluid as compared to OVA sensitized group but berberine is less effective than dexamethasone. Keywords: berberine, dexamethasone, asthma, airway inflammation How to cite: Zaidi T.S., Kausar R., Malik M., Sarfraz J., Shafiq A., Chiragh S. Comparison of berberine and dexamethasone on blood and bronchial inflammatory cells of ovalbumin sensitized guinea pigs. Esculapio 202;17(01):34-38

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小檗碱与地塞米松对卵清蛋白致敏豚鼠血液及支气管炎症细胞的影响比较

目的：比较黄连素和地塞米松对卵清蛋白致敏豚鼠的抗炎作用。方法：本实验对照研究于2016年4月在拉合尔研究生医学院进行。选择24只健康豚鼠进行研究。每组随机分配6个；正常对照组、卵清蛋白（OVA）致敏组、黄连素治疗组和地塞米松治疗组。除正常对照组外，各组在第0天和第14天通过腹膜途径注射OVA并在第25天、第26天和第27天通过吸入诱导气道炎症。黄连素治疗组和地塞米松治疗组分别在每次试验前30分钟通过腹膜途径引入黄连素（1.8mg/kg）和地塞米松（20mg/kg）。结果：黄连素治疗组（9990±1346mm3）和地塞米松治疗组（9054±1432mm3）的总白细胞计数（TLC）显著低于OVA致敏组（14261±3151mm3）。黄连素治疗组（384±26mm3）和地塞米松治疗组（306±86）mm3的支气管肺泡灌洗液TLC显著低于OVA致敏组（598±110mm3）。黄连素治疗组（21.50±3.08）和地塞米松治疗组（13.33±5.65）的血嗜酸性粒细胞百分比显著低于OVA致敏组（30.33±6.74），而黄连素处理组和地塞米松处理组的BAL液中嗜酸粒细胞百分比分别为26.00±6.69和21.00±7.46，与OVA致敏感组的40.00±7.79有显著差异。结论：与OVA致敏组相比，黄连素和地塞米松均能降低血液和BAL液中的TLC和嗜酸性粒细胞百分比，但黄连素的作用不如地塞米松。关键词：黄连素、地塞米松、哮喘、气道炎症如何引用：Zaidi T.S.、Kausar R.、Malik M.、Sarfraz J.、Shafiq A.、Chiragh S.黄连素和地塞米松对卵清蛋白致敏豚鼠血液和支气管炎症细胞的影响比较。Esculapio 202；17（01）：34-38

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Esculapio

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12 weeks