Influence of octreotide on apoptosis and metabolome expression in lipopolysaccharide-induced A549 cells

IF 1.3 3区化学 Q3 CHEMISTRY, ORGANIC

Heterocyclic Communications Pub Date : 2023-01-01 DOI:10.1515/hc-2022-0159

Luoyang Ruan, Xiaomeng Xu, Jinliang Cao, Xiaohong Xu

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Abstract

Abstract Lung cancer shows a high rate of incidence and mortality. This study aimed to investigate the influence of octreotide (OCT) on lipopolysaccharide (LPS)-induced apoptosis and metabolome expression in A549 cells. After A549 cells were treated by LPS or co-cultured with LPS and OCT, cell apoptosis was tested by flow cytometry, and gas chromatography-mass spectrometer (GC/MS) and high-performance liquid chromatography-mass spectrometer (LC/MS) were subjected to determine the differently expressed metabolites, and the interaction network was constructed. Results found that OCT promoted the apoptosis of A549 cells and significantly affected LPS-regulated cell apoptosis. Following the further investigation by orthogonal partial least squares discriminant analysis (OPLS-DA), the metabolites with different expression levels were obtained, and most of which belong to amino acids, phospholipids, organic acid, and saccharides. Fourteen components with the role of the marker metabolites included serotonin, indole, threonine, serine, glucose, phenylalanine, lactose, fumarate, 4-hydroxyphenyllactate, aspartate, asparagine, putrescine, proline, and succinate. It is indicated that OCT promotes apoptosis through five metabolism pathways and 14 key metabolism elements in A549 cells.

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奥曲肽对脂多糖诱导的A549细胞凋亡及代谢组表达的影响

摘要癌症具有较高的发病率和死亡率。本研究旨在探讨奥曲肽（OCT）对脂多糖（LPS）诱导的A549细胞凋亡和代谢组表达的影响。A549细胞经LPS处理或与LPS和OCT共培养后，用流式细胞术检测细胞凋亡，用气相色谱-质谱仪（GC/MS）和高效液相色谱-质量仪（LC/MS）测定不同表达的代谢产物，构建相互作用网络。结果发现OCT促进A549细胞凋亡，并显著影响LPS调节的细胞凋亡。通过正交偏最小二乘判别分析（OPLS-DA）进一步研究，获得了不同表达水平的代谢产物，其中大部分属于氨基酸、磷脂、有机酸和糖类。具有标记代谢产物作用的14种成分包括血清素、吲哚、苏氨酸、丝氨酸、葡萄糖、苯丙氨酸、乳糖、富马酸盐、4-羟基苯基乳酸、天冬氨酸、天冬酰胺、腐胺、脯氨酸和琥珀酸。结果表明，OCT通过5种代谢途径和14种关键代谢元素促进A549细胞凋亡。

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来源期刊

Heterocyclic Communications 化学-有机化学

CiteScore

3.80

自引率

4.30%

发文量

审稿时长

1.4 months

期刊介绍： Heterocyclic Communications (HC) is a bimonthly, peer-reviewed journal publishing preliminary communications, research articles, and reviews on significant developments in all phases of heterocyclic chemistry, including general synthesis, natural products, computational analysis, considerable biological activity and inorganic ring systems. Clear presentation of experimental and computational data is strongly emphasized. Heterocyclic chemistry is a rapidly growing field. By some estimates original research papers in heterocyclic chemistry have increased to more than 60% of the current organic chemistry literature published. This explosive growth is even greater when considering heterocyclic research published in materials science, physical, biophysical, analytical, bioorganic, pharmaceutical, medicinal and natural products journals. There is a need, therefore, for a journal dedicated explicitly to heterocyclic chemistry and the properties of heterocyclic compounds.