Effects of bioactive substances isolated from Siberian medicinal plants on the lifespan of Caenorhabditis elegans

IF 1.3 Q4 FOOD SCIENCE & TECHNOLOGY

Foods and Raw Materials Pub Date : 2022-09-23 DOI:10.21603/2308-4057-2022-2-544

Elizaveta Faskhutdinova, A. Sukhikh, V. Le, V. Minina, Mohammed El Amine Khelef, A. Loseva

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引用次数: 6

Abstract

Medicinal plants are sources of natural antioxidants. Acting as reducing agents, these substances protect the human body against oxidative stress and slow down the aging process. We aimed to study the effects of bioactive substances isolated from medicinal plants on the lifespan of Caenorhabditis elegans L. used as a model organism. High-performance liquid chromatography was applied to isolate bioactive substances from the extracts of callus, suspension, and root cultures of meadowsweet (Filipendula ulmaria L.), ginkgo (Ginkgo biloba L.), Baikal skullcap (Scutellaria baicalensis L.), red clover (Trifolium pretense L.), alfalfa (Medicágo sativa L.), and thyme (Thymus vulgaris L.). Their effect on the lifespan of C. elegans nematodes was determined by counting live nematodes treated with their concentrations of 10, 50, 100, and 200 µmol/L after 61 days of the experiment. The results were recorded using IR spectrometry. The isolated bioactive substances were at least 95% pure. We found that the studied concentrations of trans-cinnamic acid, baicalin, rutin, ursolic acid, and magniferin did not significantly increase the lifespan of the nematodes. Naringenin increased their lifespan by an average of 27.3% during days 8–26. Chlorogenic acid at a concentration of 100 µmol/L increased the lifespan of C. elegans by 27.7%. Ginkgo-based kaempferol and quercetin, as well as red clover-based biochanin A at the concentrations of 200, 10, and 100 µmol/L, respectively, increased the lifespan of the nematodes by 30.6, 41.9, and 45.2%, respectively. The bioactive substances produced from callus, root, and suspension cultures of the above medicinal plants had a positive effect on the lifespan of C. elegans nematodes. This confirms their geroprotective properties and allows them to be used as anti-aging agents.

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西伯利亚药用植物分离活性物质对秀丽隐杆线虫寿命的影响

药用植物是天然抗氧化剂的来源。这些物质作为还原剂，保护人体免受氧化应激，减缓衰老过程。我们旨在研究从药用植物中分离的生物活性物质对作为模式生物的秀丽隐杆线虫寿命的影响。采用高效液相色谱法从桔梗、银杏、黄芩、红三叶草、紫花苜蓿、，和百里香（Thymus vulgaris L.）。在实验61天后，通过计数用10、50、100和200µmol/L浓度处理的活线虫来测定它们对秀丽隐杆线虫寿命的影响。使用红外光谱法记录结果。分离的生物活性物质纯度至少为95%。我们发现，所研究的反式肉桂酸、黄芩苷、芦丁、熊果酸和大黄素的浓度并没有显著延长线虫的寿命。Naringenin使它们的寿命在第8-26天平均延长了27.3%。浓度为100µmol/L的绿原酸使秀丽隐杆线虫的寿命延长了27.7%。浓度为200、10和100µmol/L时，银杏基山奈酚和槲皮素以及红三叶草基生物炭素a分别使线虫的寿命增加了30.6%、41.9%和45.2%。从上述药用植物的愈伤组织、根和悬浮培养物中产生的生物活性物质对秀丽隐杆线虫的寿命有积极影响。这证实了它们的促性腺激素保护特性，并使它们可以用作抗衰老剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Foods and Raw Materials FOOD SCIENCE & TECHNOLOGY-

CiteScore

3.70

自引率

20.00%

发文量

审稿时长

24 weeks

期刊介绍： The journal «Foods and Raw Materials» is published from 2013. It is published in the English and German languages with periodicity of two volumes a year. The main concern of the journal «Foods and Raw Materials» is informing the scientific community on the works by the researchers from Russia and the CIS, strengthening the world position of the science they represent, showing the results of perspective scientific researches in the food industry and related branches. The main tasks of the Journal consist the publication of scientific research results and theoretical and experimental studies, carried out in the Russian and foreign organizations, as well as on the authors'' personal initiative; bringing together different categories of researchers, university and scientific intelligentsia; to create and maintain a common space of scientific communication, bridging the gap between the publications of regional, federal and international level.