Effect of Acupoint Catgut Embedding on Intestinal Flora in Rats with Ovariectomized Osteoporosis

IF 0.2 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Acupuncture & Electro-Therapeutics Research Pub Date : 2020-08-12 DOI:10.21203/rs.3.rs-52205/v1

Na Shi, Chuqiong Zhang, Long Han, Gang Ouyang

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引用次数: 0

Abstract

Background: To evaluate the effect of acupoint embedding on intestinal flora in rats with ovariectomized osteoporosis. Methods: Thirty-two 3-month-old female SD rats were randomized into four groups: Group A without operation or treatment, Group B treated with the sham operation, Group C treated with the operation and without any treatment, and Group E treated with operation and acupoint embedding therapy. The osteoporosis animal model was generated by removing bilateral ovaries of rats, and unilateral "Shenshu"(BL23) and "Zusanli" (ST36) of rats were selected for embedding therapy every time. The therapy was performed once every 1 week for 12 consecutive weeks. Results: The bone mineral density of femur and tibia and the estrogen levels in Group E are statistically higher than those in Group C (p<0.05). The Beta diversity analysis shows the difference in the diversity and abundance of flora between Group A and Group C is statistically significant (p<0.01). In Group E, the abundance of probiotics such as firmicutes and bacteroidetes at the phylum level, lactobacillaceae, lachnospiraceae, muribaculaceae, and bacteroidaceae at the family level, lactobacillus gasseri, lactobacillus reuteri, lactobacillus intestinalis, and bacteroides acidifaciens at the species level is higher than those in Group C. The contents of short-chain fatty acid in Group E are significantly higher than those in Group C (p<0.05). Conclusion: Acupoint catgut embedding can improve bone density and increase the estrogen level in rats with osteoporosis, which maybe attributed to regulating composition and abundance of intestinal flora, and increasing the content of short-chain fatty acids.

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穴位埋线对去卵巢骨质疏松大鼠肠道菌群的影响

背景：评价穴位埋线对去卵巢骨质疏松大鼠肠道菌群的影响。方法：将32只3个月大的雌性SD大鼠随机分为4组：A组不进行手术或治疗，B组进行假手术，C组进行手术或不进行任何治疗，E组进行手术和穴位埋线治疗。通过切除大鼠双侧卵巢建立骨质疏松动物模型，每次选择大鼠单侧“肾俞”（BL23）和“足三里”（ST36）进行包埋治疗。治疗每1周进行一次，连续12周。结果：E组股骨、胫骨骨密度和雌激素水平均高于C组（p<0.05）。β多样性分析显示，A组和C组菌群多样性和丰度差异具有统计学意义（p<0.01），乳酸杆菌科、lachnospiraceae科、muribaculaceae科和拟杆菌科，加氏乳杆菌、路氏乳酸杆菌、肠内乳杆菌和酸性拟杆菌在种水平上高于C组。结论：穴位埋线能改善骨质疏松大鼠骨密度，提高雌激素水平，可能与调节肠道菌群组成和丰度，增加短链脂肪酸含量有关。

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来源期刊

Acupuncture & Electro-Therapeutics Research 医学-全科医学与补充医学

CiteScore

0.50

自引率

66.70%

发文量

审稿时长

>12 weeks

期刊介绍： The aim of the journal is to provide an international forum for the exchange of ideas and promotion of basic and clinical research in acupuncture, electro-therapeutics, and related fields. The journal was established in order to make acupuncture and electro-therapeutics a universally acceptable branch of medicine through multidisciplinary research based on scientific disciplines. The final goal is to provide a better understanding of both the beneficial and adverse effects of these treatments in order to supplement or improve existing methods of diagnosis, prognosis, treatment, and prevention of diseases in both Western and Oriental medicine.