Identification of genetic variations related to pathogenicity by whole genome sequencing of Listeria monocytogenes SMFM2019-FV16 isolated from enoki mushroom

IF 1.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Food Safety Pub Date : 2023-07-04 DOI:10.1111/jfs.13076

Suyoun Choi, Yukyung Choi, Yeongeun Seo, Soomin Lee, Yohan Yoon

{"title":"Identification of genetic variations related to pathogenicity by whole genome sequencing of Listeria monocytogenes SMFM2019-FV16 isolated from enoki mushroom","authors":"Suyoun Choi, Yukyung Choi, Yeongeun Seo, Soomin Lee, Yohan Yoon","doi":"10.1111/jfs.13076","DOIUrl":null,"url":null,"abstract":"In this study, the characteristics of Listeria monocytogenes isolated from enoki mushroom (Flammulina velutipes) collected from May to July 2019 were analyzed. The isolates were analyzed for strain characteristics, hemolysis, antibiotic resistance, heat resistance, and virulence genes (inlA, inlB, actA, plcB, and hlyA) were analyzed, and whole genome sequencing (WGS) analysis was performed for the strain with the highest risk. Seven isolates were obtained from some enoki mushroom samples. All isolates were β-hemolytic and had virulence genes. Antibiotic resistance test showed multi-drug resistance in L. monocytogenes strain SMFM2019-FV15 and SMFM2019-FV16. L. monocytogenes SMFM2019-FV16 showed higher heat resistance than the other isolates. According to the results L. monocytogenes SMFM2019-FV16 was selected as the strain with the highest risk. WGS for L. monocytogenes SMFM2019-FV16 showed that it had 14 antibiotic resistance genes and 43 virulence genes, and 35 single nucleotide variants in the 43 virulence genes were observed. These results indicate that these genetic variations might be related to the high risk of L. monocytogenes SMFM2019-FV16.","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Safety","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jfs.13076","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, the characteristics of Listeria monocytogenes isolated from enoki mushroom (Flammulina velutipes) collected from May to July 2019 were analyzed. The isolates were analyzed for strain characteristics, hemolysis, antibiotic resistance, heat resistance, and virulence genes (inlA, inlB, actA, plcB, and hlyA) were analyzed, and whole genome sequencing (WGS) analysis was performed for the strain with the highest risk. Seven isolates were obtained from some enoki mushroom samples. All isolates were β-hemolytic and had virulence genes. Antibiotic resistance test showed multi-drug resistance in L. monocytogenes strain SMFM2019-FV15 and SMFM2019-FV16. L. monocytogenes SMFM2019-FV16 showed higher heat resistance than the other isolates. According to the results L. monocytogenes SMFM2019-FV16 was selected as the strain with the highest risk. WGS for L. monocytogenes SMFM2019-FV16 showed that it had 14 antibiotic resistance genes and 43 virulence genes, and 35 single nucleotide variants in the 43 virulence genes were observed. These results indicate that these genetic variations might be related to the high risk of L. monocytogenes SMFM2019-FV16.

Abstract Image

查看原文本刊更多论文

单核细胞增生李斯特菌mfm2019‐FV16全基因组测序鉴定致病性相关遗传变异

本研究分析了从2019年5月至7月采集的金针菇（Flammulina velutipes）中分离出的李斯特菌的特征。对分离物进行了菌株特征、溶血、抗生素耐药性、耐热性和毒力基因（inlA、inlB、actA、plcB 和 hlyA）分析，并对风险最高的菌株进行了全基因组测序（WGS）分析。从一些金针菇样品中获得了 7 个分离株。所有分离株都具有β溶血性和毒力基因。抗生素耐药性测试显示，单核细胞增生酵母菌株 SMFM2019-FV15 和 SMFM2019-FV16 具有多重耐药性。与其他分离物相比，单核细胞增多性酵母菌 SMFM2019-FV16 表现出更高的耐热性。根据结果，单核细胞增生 L. SMFM2019-FV16 被选为风险最高的菌株。单核细胞增生菌 SMFM2019-FV16 的 WGS 显示，它有 14 个抗生素耐药基因和 43 个毒力基因，在这 43 个毒力基因中观察到 35 个单核苷酸变异。这些结果表明，这些基因变异可能与单核细胞增多性球菌 SMFM2019-FV16 的高风险有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Food Safety 工程技术-生物工程与应用微生物

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Food Safety emphasizes mechanistic studies involving inhibition, injury, and metabolism of food poisoning microorganisms, as well as the regulation of growth and toxin production in both model systems and complex food substrates. It also focuses on pathogens which cause food-borne illness, helping readers understand the factors affecting the initial detection of parasites, their development, transmission, and methods of control and destruction.